Nano-volume plates with excellent optical properties for fast, inexpensive crystallization screening of membrane proteins
Nano-volume plates with excellent optical properties for fast, inexpensive crystallization screening of membrane proteins
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DOI：
10.1107/s002188980301906x
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发表时间：
2003-12-01
影响因子：
6.1
通讯作者：
Caffrey, M
中科院分区：
文献类型：
--
作者：
Cherezov, V;Caffrey, M
介绍了一种简单、方便、低成本的膜蛋白结晶高通量筛选玻璃基板。该板坚固耐用，大大减少了每次结晶试验中使用的蛋白质和沉淀溶液的量，同时为检测微晶体和无色蛋白质晶体提供了优异的光学性能。该板主要用于脂质中间期膜蛋白的结晶。它们也可用于可溶性蛋白和膜蛋白的批量结晶。
A simple convenient and low- cost glass- based plate for high- throughput screening of membrane protein crystallization is described. The plates are robust and reduce dramatically the amount of protein and precipitant solution used per crystallization trial, while offering excellent optical properties for the detection of micro- crystals and crystals of colorless proteins. The plates were developed primarily for crystallization of membrane proteins in lipidic mesophases. They can also be used in batch crystallization of soluble and membrane proteins.