Genetic and antigenic characterization of H5 and H7 avian influenza viruses isolated from migratory waterfowl in Mongolia from 2017 to 2019
Genetic and antigenic characterization of H5 and H7 avian influenza viruses isolated from migratory waterfowl in Mongolia from 2017 to 2019
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DOI：
10.1007/s11262-020-01764-2
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发表时间：
2020-05
期刊：
影响因子：
1.6
通讯作者：
A. Ulaankhuu;E. Bazarragchaa;M. Okamatsu;T. Hiono;K. Bodisaikhan;Tsolmon Amartuvshin;Jargalsaikhan Tserenjav;Tsogtbaatar Urangoo;Khanui Buyantogtokh;K. Matsuno;Takanari Hattori;Tatsunari Kondoh;Masahiro Sato;Y. Takadate;Shiho Torii;Mao Isono;K. Okuya;Takeshi Saito;Nodoka Kasajima;Yurie Kida;Junki Maruyama;Manabu Igarashi;A. Takada;H. Kida;Damdinjav Batchuluun;Y. Sakoda
中科院分区：
文献类型：
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作者：
A. Ulaankhuu;E. Bazarragchaa;M. Okamatsu;T. Hiono;K. Bodisaikhan;Tsolmon Amartuvshin;Jargalsaikhan Tserenjav;Tsogtbaatar Urangoo;Khanui Buyantogtokh;K. Matsuno;Takanari Hattori;Tatsunari Kondoh;Masahiro Sato;Y. Takadate;Shiho Torii;Mao Isono;K. Okuya;Takeshi Saito;Nodoka Kasajima;Yurie Kida;Junki Maruyama;Manabu Igarashi;A. Takada;H. Kida;Damdinjav Batchuluun;Y. Sakoda
各种亚型(如H5N1、H5N6、H5N8和H7N9)的高致病性禽流感病毒(HPAIV)在家禽中的传播仍然是全球动物和公共卫生关注的问题。迁徙的水禽在这些病毒的跨国传播中发挥着重要作用。为了监测野生鸟类传播病毒的情况，2015年至2019年，蒙古对候鸟进行了禽流感主动监测。总共从蒙古国中部湖畔采集了5000份粪便样本，分离出167株甲型流感病毒。本研究鉴定了两种H5N3、四种H7N3和两种H7N7病毒。这些分离株的血凝素(HA)裂解位点的氨基酸序列表明，它们对鸡的致病性较低。系统进化分析表明，所有H5和H7病毒与最近从亚洲和欧洲野鸟中分离到的H5和H7低致病性禽流感病毒(LPAIV)密切相关。H7Nx的抗原性与典型的非致病性禽流感病毒相似。虽然在蒙古候鸟中未检测到HPAIV或与A/安徽/1/2013(H7N9)相关的LPAIV，但已发现通过野生鸟类迁徙将包括H5和H7病毒在内的AIV零星传入蒙古。因此，需要继续监测家禽和野生禽类中的H5和H7禽流感病毒，以便及早发现传播到该国的HPAIV病毒。
The circulation of highly pathogenic avian influenza viruses (HPAIVs) of various subtypes (e.g., H5N1, H5N6, H5N8, and H7N9) in poultry remains a global concern for animal and public health. Migratory waterfowls play important roles in the transmission of these viruses across countries. To monitor virus spread by wild birds, active surveillance for avian influenza in migratory waterfowl was conducted in Mongolia from 2015 to 2019. In total, 5000 fecal samples were collected from lakesides in central Mongolia, and 167 influenza A viruses were isolated. Two H5N3, four H7N3, and two H7N7 viruses were characterized in this study. The amino acid sequence at hemagglutinin (HA) cleavage site of those isolates suggested low pathogenicity in chickens. Phylogenetic analysis revealed that all H5 and H7 viruses were closely related to recent H5 and H7 low pathogenic avian influenza viruses (LPAIVs) isolated from wild birds in Asia and Europe. Antigenicity of H7Nx was similar to those of typical non-pathogenic avian influenza viruses (AIVs). While HPAIVs or A/Anhui/1/2013 (H7N9)-related LPAIVs were not detected in migratory waterfowl in Mongolia, sporadic introductions of AIVs including H5 and H7 viruses into Mongolia through the wild bird migration were identified. Thus, continued monitoring of H5 and H7 AIVs in both domestic and wild birds is needed for the early detection of HPAIVs spread into the country.