Optical coherence tomography to investigate optical properties of blood during coagulation
Optical coherence tomography to investigate optical properties of blood during coagulation
复制标题
光学相干断层扫描研究凝血过程中血液的光学特性
DOI：
10.1117/1.3615667
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发表时间：
2011-09-01
影响因子：
3.5
通讯作者：
Fu, Feifei
中科院分区：
文献类型：
--
作者：
Xu, Xiangqun;Lin, Jia;Fu, Feifei
本研究使用光学相干断层扫描 (OCT) 研究静态凝固过程中人体血液的光学特性。从反射率与深度的关系曲线中获得 OCT 信号斜率 (OCTSS) 和 1/e 光穿透深度 (d(1/e))。结果表明，OCTSS 和 d(1/e) 均能够灵敏地区分凝血过程中血液特性的各个阶段。凝血 1 小时后，HCT 为 25%、35%、45% 和 55% 时的血液样本的 OCTSS 下降了 47.0%、15.0%、13.7% 和 8.5%，d(1/e) 分别增加了 34.7%、29.4%、24.3% 和 22.9%。随着HCT从35%、45%增加到55%，与凝块形成率相关的d(1/e)与时间(S-r，x 10(-4) mm/s)的斜率从6.0+/-0.3、3.7+/-0.5下降到2.3+/-0.4。对于 25%、35%、45% 和 55% 的血液，d(1/e) 演变曲线的凝血时间 (t(c)) 估计为 1969 +/- 92 秒、375 +/- 12 秒、455 +/- 11 秒和 865 +/- 47 秒。本研究表明，d(1/e) 变化的参数（t(c) 和 S-r）具有更好的灵敏度和更小的标准偏差。此外，能够通过 OCT 参数辨别影响凝血过程中血液反向散射特性的血细胞比容。结论是 OCT 是一种量化和跟踪凝血过程中血液液体-凝胶转变的潜在技术。 (C) 2011 年光电仪器工程师协会 (SPIE)。 [DOI：10.1117/1.3615667]
This study investigates the optical properties of human blood during the coagulation process under statics using optical coherence tomography (OCT). OCT signal slope (OCTSS) and 1/e light penetration depth (d(1/e)) were obtained from the profiles of reflectance versus depth. Results showed that both OCTSS and d(1/e) were able to sensitively differentiate various stages of blood properties during coagulating. After 1 h clotting, OCTSS decreased by 47.0%, 15.0%, 13.7%, and 8.5% and d(1/e) increased by 34.7%, 29.4%, 24.3%, and 22.9% for the blood samples at HCT of 25%, 35%, 45%, and 55%, respectively. The slope of d(1/e) versus time (S-r, x 10(-4) mm/s), associated with clot formation rate decreased from 6.0 +/- 0.3, 3.7 +/- 0.5 to 2.3 +/- 0.4 with the increasing of HCT from 35%, 45%, to 55%. The clotting time (t(c)) from the d(1/e) evolution curves was estimated to be 1969 +/- 92 s, 375 +/- 12 s, 455 +/- 11 s, and 865 +/- 47 s for the blood of 25%, 35%, 45%, and 55%. This study demonstrates that the parameters (t(c) and S-r) from the variations in d(1/e) had better sensitivity and smaller standard deviation. Furthermore, blood hematocrit affecting backscattering properties of blood during coagulation was capable of being discerned by OCT parameters. It is concluded that OCT is a potential technique to quantify and follow the liquid-gel transition of blood during clotting. (C) 2011 Society of Photo-Optical Instrumentation Engineers (SPIE). [DOI: 10.1117/1.3615667]