Cutting edge:: Re-evaluating the in vivo cytokine responses of CD8+ T cells during primary and secondary viral infections
Cutting edge:: Re-evaluating the in vivo cytokine responses of CD8+ T cells during primary and secondary viral infections
复制标题
DOI：
10.4049/jimmunol.174.10.5936
复制
发表时间：
2005-05-15
影响因子：
4.4
通讯作者：
Whitton, JL
中科院分区：
文献类型：
--
作者：
Liu, F;Whitton, JL
病毒特异性CD8+T细胞在接触Ag后产生IFN-γ，如果缺乏这种细胞因子，宿主通常无法根除感染。然而，我们识别在体内主动表达这种关键效应功能的细胞的能力是有限的，因为该蛋白质是快速分泌的。在本研究中，我们描述了一种简单的方法，该方法避免了离体Ag刺激的需要，并允许对在原发性和继发性病毒感染期间在体内积极合成IFN-γ的CD8（+）T细胞进行计数。产生 IFN-γ 的 Ag 特异性初级 CD8+ T 细胞的比例在感染后 5 天达到峰值，此时 T 细胞群仍在呈指数增长。记忆细胞体内 IFN-γ 的合成呈爆炸性，在继发感染后 12 小时内达到峰值，并在此后数小时内终止。该技术在许多情况下评估体内免疫细胞活性时非常有用，包括各种非感染性（例如抗免疫）疾病。
Virus-specific CD8(+) Tcellsproduce IFN-gamma after Ag contact and, in the absence of this cytokine, the host often cannot eradicate infection. However, our ability to identify cells that are actively expressing this critical effector function in vivo is limited, because the protein is rapidly secreted. In this study, we describe a simple approach that circumvents the need for ex vivo Ag stimulation and allows the enumeration of CD8(+) T cells that are actively synthesizing IFN-gamma in vivo during primary and secondary virus infections. The proportion of Ag-specific primary CD8+ T cells producing IFN-gamma peaks at 5 days postinfection, when the T cell population is still expanding exponentially. In vivo IFN-gamma synthesis by memory cells is explosive, peaking at similar to 12 h after secondary infection and terminating hours thereafter. This technique will be useful l when evaluating in vivo immune cell activity in many situations, including a variety of noninfectious (e.g., antoimmune) diseases.