Robust Distal Tip Cell Pathfinding in the Face of Temperature Stress Is Ensured by Two Conserved microRNAS in Caenorhabditis elegans.
Robust Distal Tip Cell Pathfinding in the Face of Temperature Stress Is Ensured by Two Conserved microRNAS in Caenorhabditis elegans.
复制标题
秀丽隐杆线虫中的两个保守的microRNA确保了在温度应力下的良好远端尖端细胞途径。
DOI：
10.1534/genetics.115.179184
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发表时间：
2015-08
期刊：
影响因子：
3.3
通讯作者：
Ambros V
中科院分区：
文献类型：
--
作者：
Burke SL;Hammell M;Ambros V
生物稳健性，即生物体在遗传或环境输入变化时保持稳定状态输出的能力，对于适当的发展至关重要。MicroRNA通过其对基因和基因网络的转录后调节而参与生物鲁棒性机制。以前的研究已经说明了microRNA作为反馈回路和遗传开关的一部分，通过缓冲环境和/或内部变化导致的嘈杂基因表达来促进鲁棒性的例子。在这里，我们表明，进化上保守的microRNAs的mir-34和mir-83（同源的哺乳动物mir-29）有助于强大的迁移模式的远端细胞在秀丽隐杆线虫特异性保护免受压力，温度变化。此外，我们的研究结果表明，mir-34和mir-83可能通过潜在地靶向GTdcdc-42和β-整联蛋白pat-3来调节参与远端细胞迁移的整联蛋白信号传导。我们的研究结果表明，在整合素控制的细胞迁移，可能是通过更高的生物体保守的和平号-34和平号-83的作用。它们还提供了另一个基于microRNA的发育稳健性的例子，以应对特定的环境压力，快速的温度波动。
Biological robustness, the ability of an organism to maintain a steady-state output as genetic or environmental inputs change, is critical for proper development. MicroRNAs have been implicated in biological robustness mechanisms through their post-transcriptional regulation of genes and gene networks. Previous research has illustrated examples of microRNAs promoting robustness as part of feedback loops and genetic switches and by buffering noisy gene expression resulting from environmental and/or internal changes. Here we show that the evolutionarily conserved microRNAs mir-34 and mir-83 (homolog of mammalian mir-29) contribute to the robust migration pattern of the distal tip cells in Caenorhabditis elegans by specifically protecting against stress from temperature changes. Furthermore, our results indicate that mir-34 and mir-83 may modulate the integrin signaling involved in distal tip cell migration by potentially targeting the GTPase cdc-42 and the beta-integrin pat-3. Our findings suggest a role for mir-34 and mir-83 in integrin-controlled cell migrations that may be conserved through higher organisms. They also provide yet another example of microRNA-based developmental robustness in response to a specific environmental stress, rapid temperature fluctuations.