Dynamics of cold hardiness, supercooling and cryoprotectants in diapausing and non-diapausing pupae of the cabbage root fly, Delia radicum L.
Dynamics of cold hardiness, supercooling and cryoprotectants in diapausing and non-diapausing pupae of the cabbage root fly, Delia radicum L.
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DOI：
10.1016/0022-1910(94)00124-y
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发表时间：
1995-07
影响因子：
2.2
通讯作者：
V. Košťál;P. Šimek
中科院分区：
文献类型：
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作者：
V. Košťál;P. Šimek
甘蓝根蝇，迪利亚根蝇，以滞育状态的显头蛹（PCP）越冬，即使在夏季高温下，其发育也可能暂时停止在PCP阶段。非滞育五氯苯酚的低温驯化（5 ℃下5天）导致抗寒性增加（在-15 ℃下暴露1天或20天后仍能存活），并与海藻糖含量增加有关（从6.6微克/毫克湿重增加到10.0微克/毫克湿重）。高温静止期非滞育蛹的血淋巴渗透压（623 mOsm）和葡萄糖含量（3.4 μg/mg）均高于正常非滞育蛹（550 mOsm，1.4 μg/mg）。滞育PCP在滞育开始时表现出与非滞育PCP相同的抗寒性水平。在滞育发育的前10-30天，抗寒性迅速提高，30 - 50天达到最大值，随后又明显下降。渗透压从滞育开始时的约550 mOsm增加到860 mOsm，然后缓慢下降。海藻糖在前80天从4.7 μg/mg增加到11.2 μg/mg，然后也下降。葡萄糖浓度在滞育发育的前20天呈过渡性升高，然后突然下降，直到50天左右，并在滞育结束时重复升高。肌醇含量在滞育过程中缓慢上升，80天后达到平台期。PCP阶段的过冷能力是相似的，无论蛹的生理状态或年龄。
The cabbage root fly, Delia radicum L., overwinters as a phanerocephalic pupa (PCP) in a diapause state and its development may be temporarily stopped at the PCP stage even by high summer temperatures. Cold acclimation (5 days at 5 °C) of non-diapausing PCP resulted in an increased cold hardiness (survival of exposure to − 15 °C for 1 or 20 days) and was associated with an increase of trehalose content (from 6.6 to 10.0 μg/mg wet weight). Non-diapausing pupae in a high-temperature quiescence had a higher osmotic pressure of haemolymph (623 mOsm) and a higher glucose content (3.4 μg/mg) in comparison to normal non-diapausing pupae (550 mOsm, 1.4 μg/mg). Diapausing PCP at the start of diapause showed cold hardiness levels equal to non-diapausing PCP. Their cold hardiness rapidly increased during the first 10–30 days of diapause development attaining a maximum between days 30 and 50 followed by an obvious decrease. Osmotic pressure increased from about 550 mOsm at the start of diapause to 860 mOsm by day 80 and then dropped slowly. Trehalose increased from 4.7 to 11.2 μg/mg during the first 80 days and then declined too. Glucose showed transitional increase of concentration during the first 20 days of diapause development followed by an abrupt drop until about day 50 and repeated the increase toward the diapause termination. Myo-inositol level increased slowly during diapause development showing a plateau after 80 days. Supercooling capacity of the PCP stage was similar irrespective of the physiological state or age of the pupa.