Blood-filled spaces with and without deproteinized bone grafts in guided bone regeneration - A histomorphometric study of the rabbit skull using non-resorbable membrane
Blood-filled spaces with and without deproteinized bone grafts in guided bone regeneration - A histomorphometric study of the rabbit skull using non-resorbable membrane
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DOI：
10.1111/j.1600-0501.2004.01095.x
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发表时间：
2005-04-01
影响因子：
4.3
通讯作者：
Ooya, K
中科院分区：
文献类型：
--
作者：
Okazaki, K;Shimizu, Y;Ooya, K
该实验研究评估了脱蛋白骨移植对引导骨再生（GBR）的影响。在头骨外皮质板的骨髓中制作一个凹槽。将不可吸收膜的圆顶放置在凹槽上并用钛销固定。隐蔽的移植空间充满自体血凝块（对照组）和去蛋白骨颗粒（实验组）。手术后2、4、8和12周处死兔子。制作脱钙并石蜡包埋的3μm厚的横向切片并用苏木精和曙红染色。在从皮质板到穹顶顶部的基底、中央和外围区域中，通过组织形态计量学测量新形成的骨和新形成的骨移植颗粒接触表面的比例。对照组的基底面积在4周时显着增加（P < 0.01），在8周时显着减少（P < 0.01）。中心和周边区域显示新形成骨的比例逐渐增加。实验组4周时基底区明显增加，8周时中央区和周边区明显增加（P < 0.01）。两组基底面积和中心面积差异有统计学意义（P < 0.01）。新形成的骨移植颗粒接触长度的比例在4周时显着增加（P < 0.01），而在8周和12周时三个区域没有显着下降。目前的研究表明，脱蛋白骨移植物在GBR期间可以在大范围内长时间维持新形成的骨。
This experimental study evaluated the effects of deproteinized bone grafts on guided bone regeneration (GBR). A groove was made in the bone marrow of the external cortical plate of the skull. A dome of non-resorbable membrane was placed on the groove and secured with titanium pins. The secluded graft space was filled with autogenous blood clots (control group) and deproteinized bone particles (experimental group). The rabbits were sacrified 2, 4, 8 and 12 weeks after the operation. Decalcified and paraffin-embedded, transverse 3-mu m-thick sections were made and stained with hematoxylin and eosin. The proportions of newly formed bone and newly formed bone-graft particle contact surfaces were histomorphometrically measured in the basal, central, and peripheral areas from the cortical plate to the top of the dome. In the control group, the basal area showed a significant increase at 4 weeks (P < 0.01) and a significant decrease at 8 weeks (P < 0.01). The central and peripheral areas showed gradual increases in the proportion of newly formed bone. The experimental group showed significant increase at 4 weeks in the basal area and at 8 weeks in central and peripheral area (P < 0.01). There were significant differences between both groups in basal and central area (P < 0.01). The proportion of newly formed bone-graft particle contact length showed significant increases at 4 weeks (P < 0.01) and no significant decreases at 8 and 12 weeks in three areas. The present study showed that deproteinized bone grafts maintain newly formed bone in extensive areas for a prolonged period during GBR.