Analysis of the 18S rRNA gene sequence of a Hepatozoon detected in two Japanese dogs
Analysis of the 18S rRNA gene sequence of a Hepatozoon detected in two Japanese dogs
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DOI：
10.1016/s0304-4017(02)00065-1
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发表时间：
2002-06-26
影响因子：
2.6
通讯作者：
Onishi, T
中科院分区：
文献类型：
--
作者：
Inokuma, HA;Okuda, M;Onishi, T
对日本2例犬肝人畜共患病（1例临床和1例亚临床）肝人畜共患病的18S rRNA基因部分序列（625 bp）进行了分析。这两个序列彼此完全相同，它们与在以色列发现的肝动物犬毒株有99%（617/625）核苷酸同源性密切相关。美洲肝虫与日本肝虫亲缘关系较远，同源性分别为94%（586/625）和91%（566/625）。在系统发育树中，日本肝动物与以色列的H. canis亲缘关系最密切，但与H. americanum和H. catasbianae差异显著。这些结果表明，在日本犬中检测到的肝虫可能是犬嗜血杆菌的一种变种，但显然与美洲嗜血杆菌不同。(C) 2002 Elsevier Science b.v.版权所有
Partial sequences of the 18S rRNA gene (625 bp) from a Hepatozoon detected in two canine hepatozoonosis cases, one clinical and one subclinical, in Japan were analyzed. Both sequences were identical to each other and they were closely related to the Hepatozoon canis strain found in Israel with 99% (617/625) nucleotide identity. Both Hepatozoon americanum and Hepatozoon catasbianae were distantly related to the Japanese Hepatozoon with 94% (586/625) and 91% (566/625) identities, respectively. In a phylogenetic tree, the Japanese Hepatozoon was most closely related to H. canis from Israel but was significantly different than H. americanum and H. catasbianae. These results suggest that the Hepatozoon detected in the Japanese dogs might be a strain variant of H. canis, but is apparently a different species than H. americanum. (C) 2002 Elsevier Science B.V All rights reserved.