Functional reconstitution of a bacterial CO(2) concentrating mechanism in Escherichia coli.
Functional reconstitution of a bacterial CO(2) concentrating mechanism in Escherichia coli.
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DOI：
10.7554/elife.59882
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发表时间：
2020-10-21
期刊：
影响因子：
7.7
通讯作者：
Savage DF
中科院分区：
文献类型：
--
作者：
Flamholz AI;Dugan E;Blikstad C;Gleizer S;Ben-Nissan R;Amram S;Antonovsky N;Ravishankar S;Noor E;Bar-Even A;Milo R;Savage DF
许多光合生物采用CO2浓缩机制（CCM）来通过卡尔文循环增加CO2固定速率。CCM催化全球50%的光合作用，但目前尚不清楚哪些基因和蛋白质需要产生这种复杂的适应。我们描述了在非天然宿主中构建功能性CCM，通过在大肠杆菌菌株中表达来自自养细菌的基因来实现，所述大肠杆菌菌株被工程化以依赖于rubisco羧化来生长。20个CCM基因的表达使E.大肠杆菌通过将环境空气中的CO2固定到生物质中来生长，环境空气中的生长取决于CCM的组分。因此，细菌CCM在遗传上是紧凑的，易于移植，使它们在不同细菌中的存在合理化。重组使遗传实验能够完善我们对CCM的理解，从而为支持不同生物体中CO2同化的细胞生物学的深入研究和工程奠定了基础。
Many photosynthetic organisms employ a CO2 concentrating mechanism (CCM) to increase the rate of CO2 fixation via the Calvin cycle. CCMs catalyze ≈50% of global photosynthesis, yet it remains unclear which genes and proteins are required to produce this complex adaptation. We describe the construction of a functional CCM in a non-native host, achieved by expressing genes from an autotrophic bacterium in an Escherichia coli strain engineered to depend on rubisco carboxylation for growth. Expression of 20 CCM genes enabled E. coli to grow by fixing CO2 from ambient air into biomass, with growth in ambient air depending on the components of the CCM. Bacterial CCMs are therefore genetically compact and readily transplanted, rationalizing their presence in diverse bacteria. Reconstitution enabled genetic experiments refining our understanding of the CCM, thereby laying the groundwork for deeper study and engineering of the cell biology supporting CO2 assimilation in diverse organisms.