Hepatocyte morphology and kinetics after portal vein embolization
Hepatocyte morphology and kinetics after portal vein embolization
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DOI：
10.1002/bjs.5332
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发表时间：
2006-06-01
影响因子：
9.6
通讯作者：
Nimura, Y.
中科院分区：
文献类型：
--
作者：
Komori, K.;Nagino, M.;Nimura, Y.
背景：门静脉栓塞（PVE）后的宏观体积变化可以通过计算机断层扫描准确评估，但组织学变化仍然知之甚少。本研究的目的是阐明PVE后肝细胞的形态和动力学。方法：采用肝细胞石蜡I染色对25例PVE后接受扩大肝切除术的患者的切除肝脏和5例正常肝脏进行检查，进行肝细胞组织形态学分析。通过 Ki-67 染色和末端脱氧核糖核苷酸转移酶介导的 dUTP-地高辛缺口末端标记测定法测定细胞动力学。 CD68免疫染色检测库普弗细胞。结果：栓塞肝叶、非栓塞肝叶和正常肝中肝细胞数量相似，但非栓塞肝细胞体积大于栓塞肝叶（P=0.017）。非栓塞肺叶的 Ki-67 标记指数较高（P < 0.001），而栓塞肺叶的细胞凋亡指数较高（P < 0.001）。栓塞肝叶单位面积内库普弗细胞数量较多（P < 0.001）。结论：肝细胞肥大和复制导致非栓塞肝叶体积增大，肝细胞萎缩和凋亡导致栓塞肝叶体积减小。
Background: Macroscopic volume changes after portal vein embolization (PVE) can be assessed accurately by computed tomography, but histological changes remain poorly understood. The aim of this study was to clarify hepatocyte morphology and kinetics after PVE.Methods: The resected livers from 25 patients who underwent extended hepatectomy after PVE and five normal livers were examined using hepatocyte paraffin I staining for histomorphometric analysis of hepatocytes. Cell kinetics were determined by Ki-67 staining and terminal deoxyribonucleotidyl transferase-mediated dUTP-digoxigenin nick-end labelling assay. Kupffer cells were examined by CD68 immunostaining.Results: The number of hepatocytes was similar in the embolized lobe, non-embolized lobe and normal liver, but hepatocyte volume was greater in the non-embolized lobe than in the embolized lobe (P = 0.017). The Ki-67 labelling index was higher in the non-embolized lobe (P < 0.001) whereas the apoptotic index was higher in the embolized lobe (P < 0.001). There were more Kupffer cells per unit area in the embolized lobe (P < 0.001).Conclusion: Hepatocyte hypertrophy and replication leads to volume enlargement of the non-embolized hepatic lobe, whereas hepatocyte atrophy and apoptosis causes a decrease in volume of the embolized lobe.