A Microfabricated Platform to Measure and Manipulate the Mechanics of Engineered Cardiac Microtissues
A Microfabricated Platform to Measure and Manipulate the Mechanics of Engineered Cardiac Microtissues
复制标题
DOI：
10.1089/ten.tea.2011.0341
复制
发表时间：
2012-05-01
影响因子：
4.1
通讯作者：
Chen, Christopher S.
中科院分区：
文献类型：
--
作者：
Boudou, Thomas;Legant, Wesley R.;Chen, Christopher S.
工程心肌组织可用于阐明心肌生物学的基本特征，开发体外器官型模型系统，并作为工程组织构建物在体内替代受损的心脏组织。然而，一个关键的限制是无法测试大范围的参数（细胞来源、机械、可溶性和电刺激），这些参数可能以高通量的方式影响工程组织，并在模拟天然心脏组织的环境中。在这里，我们使用微机电系统技术来生成嵌入三维微图案矩阵中的心脏微组织（cmt）阵列。微悬臂梁同时约束CMT收缩并实时报告CMT产生的力。我们展示了每100万个心脏细胞常规产生200个cmt的能力(
Engineered myocardial tissues can be used to elucidate fundamental features of myocardial biology, develop organotypic in vitro model systems, and as engineered tissue constructs for replacing damaged heart tissue in vivo. However, a key limitation is an inability to test the wide range of parameters (cell source, mechanical, soluble and electrical stimuli) that might impact the engineered tissue in a high-throughput manner and in an environment that mimics native heart tissue. Here we used microelectromechanical systems technology to generate arrays of cardiac microtissues (CMTs) embedded within three-dimensional micropatterned matrices. Microcantilevers simultaneously constrain CMT contraction and report forces generated by the CMTs in real time. We demonstrate the ability to routinely produce similar to 200 CMTs per million cardiac cells (