Postsynaptic mad signaling at the Drosophila neuromuscular junction
Postsynaptic mad signaling at the Drosophila neuromuscular junction
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DOI：
10.1016/j.cub.2006.02.061
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发表时间：
2006-04-04
期刊：
影响因子：
9.2
通讯作者：
González-Gaitán, M
中科院分区：
文献类型：
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作者：
Dudu, V;Bittig, T;González-Gaitán, M
背景资料：突触处的细胞间通讯涉及突触传递以及由生长因子介导的信号传导，生长因子提供发育和可塑性线索。有证据表明，一个逆行的，突触前转化生长因子-β（TGF-β）信号事件调节突触的发育和功能在Drosophila.Results：在这里，我们表明，突触后TGF-β信号事件发生在幼虫发育过程中。I型受体厚静脉（TcR）和R-Smad转录因子Mothers-against-dpp（Mad）位于肌肉中的突触后。此外，Mad磷酸化发生在突触后突触下网（SSR）内面向神经递质释放的突触前活性区的区域。为了在真实的时间监测突触传递期间突触中TGF-β信号传导的水平，我们建立了FRAP测定来测量肌肉中Mad核输入/输出。我们发现，疯狂核贩运依赖于刺激的muscles.Conclusions：我们的数据表明，突触传递和突触后TGF-β信号，可能协调神经肌肉的发育和功能的机制。
Background: Cell-to-cell communication at the synapse involves synaptic transmission as well as signaling mediated by growth factors, which provide developmental and plasticity cues. There is evidence that a retrograde, presynaptic transforming growth factor-beta (TGF-beta) signaling event regulates synapse development and function in Drosophila.Results: Here we show that a postsynaptic TGF-beta signaling event occurs during larval development. The type I receptor Thick veins (Tkv) and the R-Smad transcription factor Mothers-against-dpp (Mad) are localized postsynaptically in the muscle. Furthermore, Mad phosphorylation occurs in regions facing the presynaptic active zones of neurotransmitter release within the postsynaptic subsynaptic reticulum (SSR). In order to monitor in real time the levels of TGF-beta signaling in the synapse during synaptic transmission, we have established a FRAP assay to measure Mad nuclear import/export in the muscle. We show that Mad nuclear trafficking depends on stimulation of the muscle.Conclusions: Our data suggest a mechanism linking synaptic transmission and postsynaptic TGF-beta signaling that may coordinate nerve-muscle development and function.