Identification of differentially regulated maize proteins conditioning Sugarcane mosaic virus systemic infection
Identification of differentially regulated maize proteins conditioning Sugarcane mosaic virus systemic infection
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调节甘蔗花叶病毒全身感染的差异调节玉米蛋白的鉴定
DOI：
10.1111/nph.14645
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发表时间：
2017-08-01
期刊：
影响因子：
9.4
通讯作者：
Zhou, Tao
中科院分区：
文献类型：
--
作者：
Chen, Hui;Cao, Yanyong;Zhou, Tao
甘蔗花叶病毒（Sugarcane mosaic virus，SCMV）是引起玉米矮花叶病的重要病原。为了鉴定玉米对SCMV侵染的应答基因，以第一和第二次系统侵染的玉米叶片（分别称为1 SL和2 SL）为材料，进行比较蛋白质组学分析，在1 SL和2 SL中鉴定出71个SCMV侵染后差异表达的蛋白。其中8种蛋白质在1SL和2SL中的变化规律相同。调节蛋白的功能注释和光合活性的测量显示，光合作用受到更多的抑制和防御基因的表达更显着，在1 SL比2 SL。敲下的调节蛋白在1 SL和2 SL的雀麦花叶病毒为基础的基因沉默载体在玉米中表明，蛋白质二硫键异构酶样和磷酸甘油酸激酶所需的最佳SCMV复制。相比之下，多胺氧化酶（ZmPAO）的敲除显着增加了SCMV的积累，这意味着ZmPAO活性可能有助于抗性或耐受性。结果表明，将不同组织的比较蛋白质组学分析与病毒诱导的基因沉默相结合，是鉴定支持病毒复制或增强病毒感染抗性的宿主蛋白质的有效方法。
Sugarcane mosaic virus (SCMV) is the most important cause of maize dwarf mosaic disease. To identify maize genes responsive to SCMV infection and that may be involved in pathogenesis, a comparative proteomic analysis was performed using the first and second systemically infected leaves (termed 1 SL and 2 SL, respectively).Seventy-one differentially expressed proteins were identified in 1 SL and 2 SL upon SCMV infection. Among them, eight proteins showed the same changing patterns in both 1 SL and 2 SL. Functional annotations of regulated proteins and measurement of photosynthetic activity revealed that photosynthesis was more inhibited and defensive gene expression more pronounced in 1 SL than in 2 SL.Knockdown of regulated proteins in both 1 SL and 2 SL by a brome mosaic virus-based gene silencing vector in maize indicated that protein disulfide isomerase-like and phosphoglycerate kinase were required for optimal SCMV replication. By contrast, knockdown of polyamine oxidase (ZmPAO) significantly increased SCMV accumulation, implying that ZmPAO activity might contribute to resistance or tolerance.The results suggest that combining comparative proteomic analyses of different tissues and virus-induced gene silencing is an efficient way to identify host proteins supporting virus replication or enhancing resistance to virus infection.