Defective Leptotene Chromosome 1 (DLC1) encodes a type-B response regulator and is required for rice meiosis
Defective Leptotene Chromosome 1 (DLC1) encodes a type-B response regulator and is required for rice meiosis
复制标题
有缺陷的细线染色体 1 (DLC1) 编码 B 型反应调节因子，是水稻减数分裂所必需的
DOI：
10.1111/tpj.14344
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发表时间：
2019-08-01
期刊：
影响因子：
7.2
通讯作者：
Li, Shuangcheng
中科院分区：
文献类型：
--
作者：
Tao, Yang;Chen, Dan;Li, Shuangcheng
减数分裂对于大多数真核生物的有性生殖和新等位基因变异的产生至关重要。在这项研究中，我们报告的减数分裂基因，DLC1的分离，使用基于图位的克隆策略。dlc1突变体是不育的男性和女性配子体由于早期的缺陷，在细线染色体和随后的异常在后期阶段。DLC1在花粉母细胞（PMCs）和绒毡层中强烈表达，编码一个位于细胞核的具有转录活性的水稻B型反应调节因子（RR）。进一步的研究表明，DLC1与所有5个假定的水稻组氨酸磷酸转移蛋白（HP）在酵母和植物细胞中相互作用，这表明可能参与水稻减数分裂的双组分信号系统（TCS）。我们的研究结果表明，DLC1是水稻减数分裂和育性所必需的，为TCS在水稻减数分裂中的作用提供了有用的信息。
Meiosis is critical for sexual reproduction and the generation of new allelic variations in most eukaryotes. In this study, we report the isolation of a meiotic gene, DLC1, using a map-based cloning strategy. The dlc1 mutant is sterile in both male and female gametophytes due to an earlier defect in the leptotene chromosome and subsequent abnormalities at later stages. DLC1 is strongly expressed in the pollen mother cells (PMCs) and tapetum and encodes a nucleus-located rice type-B response regulator (RR) with transcriptional activity. Further investigations showed that DLC1 interacts with all five putative rice histidine phosphotransfer proteins (HPs) in yeast and planta cells, suggesting a possible participation of the two-component signalling systems (TCS) in rice meiosis. Our results demonstrated that DLC1 is required for rice meiosis and fertility, providing useful information for the role of TCS in rice meiosis.