microRNA-182 inhibits the proliferation and migration of glioma cells through the induction of neuritin expression
microRNA-182 inhibits the proliferation and migration of glioma cells through the induction of neuritin expression
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microRNA-182通过诱导神经突蛋白表达抑制神经胶质瘤细胞的增殖和迁移
DOI：
10.3892/ol.2015.3365
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发表时间：
2015-08-01
期刊：
影响因子：
2.9
通讯作者：
Wu, Yuncheng
中科院分区：
文献类型：
--
作者：
Feng, Ya;Liu, Te;Wu, Yuncheng
星形细胞瘤是最常见的神经胶质瘤，预后差。然而，星形细胞瘤的发病机制仍有待阐明。Neuritin是神经营养因子家族的新成员，通过调节细胞凋亡和增殖与肿瘤恶性程度相关。本研究克隆了microRNA-182（miR-182），并将其转染人星形细胞瘤细胞系U251，研究其对星形细胞瘤细胞增殖和迁移的调控作用，以及与neuritin表达的关系。结果显示，miR-182特异性靶向编码神经突素的基因NRN 1，如NRN 1的蛋白质和mRNA水平的降低所证明的。此外，过表达miR-182影响细胞周期调控和细胞迁移能力，这可能与该分子促进细胞凋亡有关。总之，内源性miR-182可能参与了星形细胞瘤的发病机制，这与miR-182调控的基因NRN 1有关。
Astrocytomas are the most common type of glial tumors and carry a poor prognosis. However, the pathogenesis of astrocytomas remains to be elucidated. Neuritin, a novel member of the neurotrophic factors family, has been shown to be associated with tumor malignancy, via the regulation of apoptosis and proliferation. In the present study, microRNA-182 (miR-182) was cloned and transfected into the U251 human astrocytoma cell line, in order to investigate its regulatory effects on the proliferation and migration of these cells, as well as its association with the expression of neuritin. The results showed that miR-182 specifically targets the gene encoding neuritin, NRN1, as demonstrated by a reduction in the protein and mRNA levels of NRN1. In addition, overexpression of miR-182 affected cell cycle regulation and cell migration capacity in vitro, which may have been associated with the promotion of apoptosis by this molecule. In conclusion, endogenous miR-182 may be involved in the pathogenesis of astrocytoma, which is associated with the miR-182-regulated gene, NRN1.