Elevated Evolutionary Rates among Functionally Diverged Reproductive Genes across Deep Vertebrate Lineages.
Elevated Evolutionary Rates among Functionally Diverged Reproductive Genes across Deep Vertebrate Lineages.
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DOI：
10.4061/2011/274975
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发表时间：
2011-01-01
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影响因子：
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通讯作者：
Kulathinal, Rob J.
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文献类型：
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作者：
Grassa, Christopher J.;Kulathinal, Rob J.
在密切相关的分类群中，参与生殖的蛋白质通常比其他蛋白质进化得更快。在这里，我们应用功能和比较基因组学的方法来比较功能的分歧，在一个深层次的系统发育阵列的产卵和活轴承脊椎动物类群。我们使用来自可用生殖组织（包括睾丸和卵巢）和非生殖组织的EST以及Gene Ontology，根据功能比对并注释了Anolis carolinensis（绿色蜥蜴）、Danio rerio（斑马鱼）、Xenopus tropicalis（青蛙）、Gallus gallus（鸡）和Mus musculus（小鼠）中的4，986个1：1：1：1直向同源物.对于每个物种谱系，基因被进一步分类为组织特异性的（在单个组织中发现）或组织表达的（在多个组织中发现）。在独立的脊椎动物谱系中，我们通常发现性腺特异性基因的进化速度比性腺表达基因快，并且比非生殖基因明显快。在性腺组中，睾丸基因通常比卵巢基因更趋分化。令人惊讶的是，在所有五个谱系中保持功能保守的直系同源物的子集中发现了相反但不显著的模式。这些功能分化和功能保守的生殖直系同源物之间发现的对比进化模式提供了证据，普遍和潜在的神秘谱系特定的选择过程中的祖先生殖系统的脊椎动物。
Among closely related taxa, proteins involved in reproduction generally evolve more rapidly than other proteins. Here, we apply a functional and comparative genomics approach to compare functional divergence across a deep phylogenetic array of egg-laying and live-bearing vertebrate taxa. We aligned and annotated a set of 4,986 1 : 1 : 1 : 1 : 1 orthologs in Anolis carolinensis (green lizard), Danio rerio (zebrafish), Xenopus tropicalis (frog), Gallus gallus (chicken), and Mus musculus (mouse) according to function using ESTs from available reproductive (including testis and ovary) and non- reproductive tissues as well as Gene Ontology. For each species lineage, genes were further classified as tissue-specific (found in a single tissue) or tissue- expressed (found in multiple tissues). Within independent vertebrate lineages, we generally find that gonadal-specific genes evolve at a faster rate than gonadal-expressed genes and significantly faster than non-reproductive genes. Among the gonadal set, testis genes are generally more diverged than ovary genes. Surprisingly, an opposite but nonsignificant pattern is found among the subset of orthologs that remained functionally conserved across all five lineages. These contrasting evolutionary patterns found between functionally diverged and functionally conserved reproductive orthologs provide evidence for pervasive and potentially cryptic lineage-specific selective processes on ancestral reproductive systems in vertebrates.