Differential effects of mineralocorticoid and angiotensin II on incentive and mesolimbic activity.
Differential effects of mineralocorticoid and angiotensin II on incentive and mesolimbic activity.
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DOI：
10.1016/j.yhbeh.2015.12.002
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发表时间：
2016-03
影响因子：
3.5
通讯作者：
Flanagan-Cato LM
中科院分区：
文献类型：
--
作者：
Grafe LA;Flanagan-Cato LM
口渴和钠食欲的控制部分是由醛固酮和血管紧张素 II (AngII) 激素介导的。本研究检查了接受全身盐皮质激素、脑室内血管紧张素II或两者治疗的动物努力值改变的行为和神经机制。首先，用盐皮质激素和 AngII 治疗的大鼠在渐进比例操作任务中进行测试。钠与水的工作意愿取决于激素治疗。特别是，与仅给予其中一种激素的大鼠相比，同时接受盐皮质激素和 AngII 治疗的大鼠更容易获取钠而不是水。其次，检查了中脑边缘多巴胺通路的组成部分是否受到盐皮质激素和 AngII 的调节。根据 cFos 免疫组织化学，AngII 治疗激活了腹侧被盖区和伏核的神经元，而盐皮质激素预处理没有增强作用。相反，蛋白质印迹分析显示，联合激素治疗增加了腹侧被盖区磷酸酪氨酸羟化酶的水平。因此，盐皮质激素和 AngII 治疗根据酪氨酸羟化酶水平与 cFos 激活不同地参与中脑边缘通路。
The controls of thirst and sodium appetite are mediated in part by the hormones aldosterone and angiotensin II (AngII). The present study examined the behavioral and neural mechanisms of altered effort-value in animals treated with systemic mineralocorticoids, intracerebroventricular AngII, or both. First, rats treated with mineralocorticoid and AngII were tested in the progressive ratio operant task. The willingness to work for sodium versus water depended on hormonal treatment. In particular, rats treated with both mineralocorticoid and AngII preferentially worked for access to sodium versus water compared with rats given only one of these hormones. Second, components of the mesolimbic dopamine pathway were examined for modulation by mineralocorticoids and AngII. Based on cFos immunohistochemistry, AngII treatment activated neurons in the ventral tegmental area and nucleus accumbens, with no enhancement by mineralocorticoid pretreatment. In contrast, western blot analysis revealed that combined hormone treatment increased levels of phospho-tyrosine hydroxylase in the ventral tegmental area. Thus, mineralocorticoid and AngII treatments differentially engaged the mesolimbic pathway based on tyrosine hydroxylase levels versus cFos activation.