Shear Stress Regulates Adhesion and Rolling of CD44+ Leukemic and Hematopoietic Progenitor Cells on Hyaluronan
Shear Stress Regulates Adhesion and Rolling of CD44+ Leukemic and Hematopoietic Progenitor Cells on Hyaluronan
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DOI：
10.1016/j.bpj.2011.05.045
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发表时间：
2011-08-03
影响因子：
3.4
通讯作者：
Rosenhahn, Axel
中科院分区：
文献类型：
--
作者：
Christophis, Christof;Taubert, Isabel;Rosenhahn, Axel
白血病细胞和表达CD44受体的人造血祖细胞具有附着和滚动透明质酸的能力。我们在微流控系统中定量研究了白血病细胞系和造血祖细胞在透明质酸和海藻酸多糖薄膜上的粘附行为。如果阈值剪应力超过0.2 dyn/cm(2)，则施加的流量会增强cd44阳性细胞与透明质酸之间的相互作用。在剪切应力为1 dyn/cm(2)时，细胞的滚动速度最高可达15 mum /s。缺乏cd44表达的白血病Jurkat和Kasumi-1细胞在透明质酸上没有粘附和滚动，而表达cd44的白血病细胞KG-la、HL-60、K-562和造血祖细胞在透明质酸上有粘附和滚动。有趣的是，流诱导细胞滚动的观察结果与白细胞向炎症部位募集和干细胞归巢到骨髓的机制有关。
Leukemic cells and human hematopoietic progenitor cells expressing CD44 receptors have the ability to attach and roll on hyaluronan. We investigated quantitatively the adhesion behavior of leukemic cell lines and hematopoietic progenitor cells on thin films of the polysaccharides hyaluronan and alginate in a microfluidic system. An applied flow enhances the interaction between CD44-positive cells and hyaluronan if a threshold shear stress of 0.2 dyn/cm(2) is exceeded. At shear stress similar to 1 dyn/cm(2), the cell rolling speed reaches a maximum of 15 mu m/s. Leukemic Jurkat and Kasumi-1 cells lacking CD44-expression showed no adhesion or rolling on the polysaccharides whereas the CD44-expressing leukemic cells KG-la, HL-60, K-562, and hematopoietic progenitor cells attached and rolled on hyaluronan. Interestingly, the observations of flow-induced cell rolling are related to those found in the recruitment of leukocytes to inflammatory sites and the mechanisms of stem-cell homing into the bone marrow.