FT-IR MICROSCOPIC MAPPINGS OF EARLY MINERALIZATION IN CHICK LIMB BUD MESENCHYMAL CELL-CULTURES
FT-IR MICROSCOPIC MAPPINGS OF EARLY MINERALIZATION IN CHICK LIMB BUD MESENCHYMAL CELL-CULTURES
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DOI：
10.1007/bf00296678
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发表时间：
1992-12-01
影响因子：
4.2
通讯作者：
BINDERMAN, I
中科院分区：
文献类型：
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作者：
BOSKEY, AL;CAMACHO, NP;BINDERMAN, I
鸡肢芽间充质细胞在培养中分化为软骨细胞并形成软骨基质。在这项研究中，矿物形成在不同地区内的文化补充4 mM无机磷酸盐，或2.5，5.0，和10 mM β-甘油磷酸盐（betaGP），其特征在于傅立叶变换红外（FT-IR）显微镜。从第14天到第30天测量相对矿物与基质的比率以及整个基质中特定位置处的晶体尺寸分布。检测到的唯一矿物相是结晶不良的磷灰石。接受4 mM无机磷酸盐的培养物具有较小的晶体，其在软骨结节周围的随机分布比β GP处理的培养物中的晶体少。BetaGP诱导的矿物由更大、更完美的磷灰石晶体组成。在接受5或10 mM betaGP的培养物中，相对矿物质-基质比（分别从磷酸盐和酰胺I带的积分强度计算）高于4 mM无机磷酸盐培养物或体内钙化鸡软骨。
Chick limb bud mesenchymal cells differentiate into chondrocytes and form a cartilaginous matrix in culture. In this study, the mineral formed in different areas within cultures supplemented with 4 mM inorganic phosphate, or 2.5, 5.0, and 10 mM beta-glycerophosphate (betaGP), was characterized by Fourier-transform infrared (FT-IR) microscopy. The relative mineral-to-matrix ratios, and distribution of crystal sizes at specific locations throughout the matrix were measured from day 14 to day 30. The only mineral phase detected was a poorly crystalline apatite. Cultures receiving 4 mM inorganic phosphate had smaller crystals which were less randomly distributed around the cartilage nodules than those in the betaGP-treated cultures. BetaGP-induced mineral consisted of larger, more perfect apatite crystals. In cultures receiving 5 or 10 mM betaGP, the relative mineral-to-matrix ratios (calculated from the integrated intensities of the phosphate and amide I bands, respectively) were higher than in the cultures with 4 mM inorganic phosphate or in the in vivo calcified chick cartilage.