A major gene affecting age-related hearing loss in C57BL/6J mice
A major gene affecting age-related hearing loss in C57BL/6J mice
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DOI：
10.1016/s0378-5955(97)00155-x
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发表时间：
1997-12-01
期刊：
影响因子：
2.8
通讯作者：
Zheng, QY
中科院分区：
文献类型：
--
作者：
Johnson, KR;Erway, LC;Zheng, QY
通过（C57 BL/6 J × CAST/Ei）× C57 BL/6 J回交分析，定位了C57 BL/6 J小鼠年龄相关性听力损失（阿勒）的主要基因。阿勒，通过升高的脑干反应（ABR）阈值测量，回交小鼠之间的分离，如预期的隐性，主要是单基因性状。定性和定量连锁分析均显示AHL基因（阿勒）位于第10号染色体D10 Mit 5附近的遗传图谱位置相同。然后使用标记辅助选择来产生含有10号染色体的不同CAST衍生区段的C57 BL/6 J的同源系。本文报告了这两个同源系老年祖细胞的ABR测试结果和耳蜗组织病理学。阿勒是第一个在小鼠中报道的引起迟发性、非综合征性听力损失的基因。(C)1997年Elsevier Science B.V.
A major gene responsible for age-related hearing loss (AHL) in C57BL/6J mice was mapped by analyses of a (C57BL/6J x CAST/Ei) x C57BL/6J backcross. AHL, as measured by elevated auditory-evoked brainstem response (ABR) thresholds, segregated among backcross mice as expected for a recessive, primarily single-gene trait. Both qualitative and quantitative linkage analyses gave the same genetic map position for the AHL gene (Ahl) on chromosome 10, near D10Mit5. Marker assisted selection was then used to produce congenic lines of C57BL/6J that contain different CAST-derived segments of chromosome 10. ABR test results and cochlear histopathology of aged progenitors of these congenic lines are presented. Ahl is the first gene causing late-onset, non-syndromic hearing loss that has been reported in the mouse. (C) 1997 Elsevier Science B.V.