Microglial polarization dynamics in dorsal spinal cord in the early stages following chronic sciatic nerve damage
Microglial polarization dynamics in dorsal spinal cord in the early stages following chronic sciatic nerve damage
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慢性坐骨神经损伤后早期背脊髓小胶质细胞极化动态
DOI：
10.1016/j.neulet.2016.01.038
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发表时间：
2016-03-23
影响因子：
2.5
通讯作者：
Huang, Changsheng
中科院分区：
文献类型：
--
作者：
Xu, Fangting;Huang, Juan;Huang, Changsheng
周围神经损伤可导致脊髓小胶质细胞的激活，从而介导神经炎症，导致神经损伤后的神经病理性疼痛。激活的小胶质细胞可能表现为促炎的M1表型，也可能表现为抗炎的M2表型，这可能导致神经系统中有害或有益的作用。本研究观察了坐骨神经慢性压迫损伤(CCI)后14天以上大鼠脊髓背侧小胶质细胞的数量、形态和基因表达谱。CCI后1d内，部分小胶质细胞形态由正常状态变为激活状态。神经病理性疼痛发生在损伤后7至14天内，小胶质细胞数量增加，几乎所有的小胶质细胞都在脊髓背侧被定义为激活的。CCI后第1天，M1和M2小胶质细胞相关基因均升高，第7天和第14天仅M1小胶质细胞相关基因持续升高。这些结果表明，在CCI后1天，脊髓背侧的M1和M2小胶质细胞均被激活，但在随后的7天和14天，小胶质细胞向M1表型倾斜。(C)2016爱思唯尔爱尔兰有限公司。保留所有权利。
Peripheral nerve injury can lead to activation of spinal microglia, which can mediate neuroinflammation and contribute to neuropathic pain following nerve injury. Activated microglia may manifest with either pro-inflammatory M1 phenotype or anti-inflammatory M2 phenotype, which may lead to detrimental or beneficial roles in the nervous system. In this study, microglia numbers, morphology and gene profiles were examined in the dorsal spinal cord of rats over 14 days following sciatic nerve chronic constriction injury (CCI). The morphology of some microglia changed from a surveying to an activated state within 1 day of CCI. Neuropathic pain developed within seven to 14 days following injury and microglia numbers were increased, with almost all in the dorsal spinal cord morphologically defined as activated. At day one after CCI, both M1 and M2 microglia-related genes were increased but only M1 microglia-related genes remained elevated at day seven and 14 thereafter. These results indicate that both M1 and M2 microglia were activated in the dorsal spinal cord one day after CCI but the microglia skewed towards M1 phenotype during the following seven and 14 days. (C) 2016 Elsevier Ireland Ltd. All rights reserved.