Phosphorothioate oligodeoxynucleotides are potent sequence nonspecific inhibitors of de novo infection by HIV.
Phosphorothioate oligodeoxynucleotides are potent sequence nonspecific inhibitors of de novo infection by HIV.
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硫代磷酸酯寡脱氧核苷酸是 HIV 从头感染的有效序列非特异性抑制剂。
DOI：
10.1089/aid.1989.5.639
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发表时间：
1989
影响因子：
1.5
通讯作者：
J. Cohen
中科院分区：
文献类型：
--
作者：
C. Stein;M. Matsukura;C. Subasinghe;S. Broder;J. Cohen
最近发现硫代磷酸同源寡脱氧核苷酸可以保护ATH-8细胞免受HIV从头感染的细胞病变效应。该效应是剂量和链长依赖性的，对于21-28-mer观察到最大效应。我们现在已经合成了一系列具有混合序列的硫代磷酸酯寡聚体，并发现它们都具有剂量依赖性细胞保护作用，在约1-2 μ M的寡聚体浓度下最大。最不有效的序列仅含有A或T，而最有效的序列具有40%或更高的GC含量。结果还证实了长度效应，即21-mers比14-mers更具有细胞保护性。
Phosphorothioate homo-oligodeoxynucleotides have recently been found to protect ATH-8 cells against the cytopathic effect of de novo infection by HIV. The effect is dose and chain-length dependent, with a maximum effect seen for 21-28-mers. We have now synthesized a series of phosphorothioate oligomers with mixed-based sequences and found that all of them have a dose-dependent cytoprotective effect that is maximal at an oligomer concentration of about 1-2 microM. The least effective sequences contain only A or T, and the most effective sequences have 40% GC content or greater. The results also confirm the length effect, namely that 21-mers are more cytoprotective than 14-mers.