Alternative splicing of papilin and the diversity of Drosophila extracellular matrix during embryonic morphogenesis
Alternative splicing of papilin and the diversity of Drosophila extracellular matrix during embryonic morphogenesis
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DOI：
10.1002/dvdy.10265
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发表时间：
2003-04-01
影响因子：
2.5
通讯作者：
Fessler, JH
中科院分区：
文献类型：
--
作者：
Kramerova, IA;Kramerov, AA;Fessler, JH
乳蝶蛋白是细胞外基质蛋白，它们具有特定的、共同的蛋白质结构域类型顺序。它们广泛存在，从线虫到人类，并且给定类型结构域的重复次数可能不同。前体 mRNA 的差异剪接增加了蛋白质的多样性。我们报告说，果蝇具有紧凑的基因组，在胚胎发生过程中以发育定义的模式表达乳头蛋白的三种剪接变体。这些亚型具有不同数量的 Kunitz 和 IgC2 结构域。乳头状蛋白亚型在特定细胞类型中表达，并有助于原肠胚形成褶皱、早期中胚层、心脏形成、基底膜以及肠道外周营养膜的形成中的不同细胞外基质。这一发现表明果蝇胚胎中存在出乎意料的广泛的不同细胞周基质。这种含有乳头蛋白的基质具有发育和功能意义，正如我们之前表明的那样，乳头蛋白合成的抑制和异位过度表达都会致命地破坏器官发生。 (C) 2003 Wiley-Liss, Inc.
Papilins are extracellular matrix proteins that share a particular, common order of types of protein domains. They occur widely, from nematodes to man, and can differ in the number of repeats of a given type of domain. Protein variety is increased by differential splicing of pre-mRNA. We report that Drosophila, which has a compact genome, expresses three splice variants of papilin during embryogenesis in developmentally defined patterns. These isoforms have different numbers of Kunitz and IgC2 domains. The papilin isoforms are expressed in specific cell types and contribute to different extracellular matrices in gastrulation folds, early mesoderm, heart formation, basement membranes, and elaboration of the excorporeal peritrophic membrane that lines the gut. This finding indicates an unexpectedly broad spectrum of different pericellular matrices in Drosophila embryos. Such papilin-containing matrices have developmental as well as functional significance, as we previously showed that both suppression of papilin synthesis and ectopic overexpression lethally disrupt organogenesis. (C) 2003 Wiley-Liss, Inc.