Nucleotide and deduced amino acid sequences of the nominal nonstructural phosphoprotein of the ERA, PM and CVS-11 strains of rabies virus.
Nucleotide and deduced amino acid sequences of the nominal nonstructural phosphoprotein of the ERA, PM and CVS-11 strains of rabies virus.
复制标题
狂犬病病毒 ERA、PM 和 CVS-11 株名义非结构磷蛋白的核苷酸序列和推导的氨基酸序列。
DOI：
10.1093/nar/18.23.7172
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发表时间：
1990
影响因子：
14.9
通讯作者：
Wunner,WH
中科院分区：
文献类型：
--
作者：
Larson,JK;Wunner,WH
狂犬病病毒的非结构磷蛋白是病毒核糖核蛋白复合物的组成部分。通过类推，对于水泡性口炎病毒的NS蛋白，弹状病毒科的原型，包括狂犬病病毒，狂犬病病毒的NS蛋白是单链RNA基因组的转录和复制所必需的（1）。先前已报道了狂犬病病毒巴斯德株NS基因的核苷酸序列和推导的氨基酸序列（2）。我们已经对另外三种实验室适应的狂犬病病毒株的NS基因进行了测序：EvelynRokitnicki-Abelseth（ERA）株、Pitman-Moore（PM）株和攻击病毒标准11（CVS-11）株。ERA菌株的NS基因已被克隆（3），并通过双脱氧链终止法（4）和化学切割法（5）对cDNA进行了完整测序。通过使用NS特异性寡核苷酸引物对ERA的单链RNA基因组进行直接测序来确认核苷酸序列，以通过包含NS基因的基因组区域进行测序。还通过直接测序病毒的基因组RNA获得来自PM和CVS-11菌株的核苷酸序列（6）。使用基因组RNA作为双脱氧测序模板的优势在于能够从同质群体而不是从可能含有错误掺入的核苷酸的cDNA克隆中获得准确的数据。本报告中的序列以mRNA形式呈现，其是负义RNA基因组的互补序列，并涵盖NS mRNA转录起始位点（5 '-AACA-3'）和假定的病毒多聚腺苷酸化信号（5 '-TGAAAAAAA-3'）之间的所有核苷酸。编码297个氨基酸残基的开放阅读框位于起始于核苷酸30的起始密码子（ATG）和终止于核苷酸923的终止密码子（TAA）之间。ERA和PM株的核苷酸和推导的氨基酸序列在整个该区域内是100%同源的。先前报道的巴斯德菌株的NS序列（2）与ERA和PM序列在核苷酸水平上显示99%的同源性，在氨基酸水平上显示98%的同源性。CVS-11株的核苷酸序列与ERA株和PM株的同源性为88%，但在氨基酸水平上显示92%的同源性。
The nominal nonstructural (NS) phosphoprotein of rabies virus is an integral component of the viral ribonucleoprotein complex. By analogy, to the NS protein of the vesicular stomatitis virus, the prototype of the Rhabdoviridae family which includes the rabies virus, the NS protein of the rabies virus is required for the transcription and replication of the single-stranded RNA genome (1). The nucleotide sequence and deduced amino acid sequence of the NS gene of the Pasteur strain of rabies virus has been reported previously (2). We have sequenced the NS genes of three additional laboratory-adapted strains of rabies virus: EvelynRokitnicki-Abelseth (ERA) strain, Pitman-Moore (PM) strain, and challenge virus standard-I 1 (CVS-1 1) strain. The NS gene of the ERA strain has been cloned (3), and the cDNA was sequenced in its entirety by the dideoxy chain termination method (4) and intermittently by the chemical cleavage method (5). The nucleotide sequence was confirmed by direct sequencing of the single-stranded RNA genome of ERA using NS-specific oligonucleotide primersto processively sequence through the region of the genome containing the NS gene. Nucleotide sequences from the PM and CVS-1 1 strains were also obtained by directly sequencing the genomic RNA of the viruses (6). The advantage of using genomic RNA as the template for dideoxy sequencing lies in the ability to obtain accurate data from a homogeneous population rather than from a cDNA clone which may contain a misincorporated nucleotide (s). The sequences in this report are presented in the mRNA sense, which is the complement of the negative-sense RNA genome, and encompass all nucleotides between the NS mRNA transcriptional start site (5'-AACA-3') and the presumptive viral polyadenylation signal (5'-TGAAAAAAA-3'). An open reading frame coding for 297 amino acid residues is located between the initiation codon (ATG) starting at nucleotide 30 and a termination codon (TAA) ending at nucleotide 923. The nucleotide and deduced amino acid sequences of the ERA and PM strains are 100% homologous throughout this region. The previously reported NS sequence of the Pasteur strain (2) displays 99% homology at the nucleotide level and 98% homology at the amino acid level to the ERA and PM sequences. The nucleotide sequence of the CVS-11 strain is 88% homologous to the ERA and PM strains, but displays 92% homology at the amino acid level.