Induction of midkine expression in reactive astrocytes following rat transient forebrain ischemia
Induction of midkine expression in reactive astrocytes following rat transient forebrain ischemia
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DOI：
10.1006/exnr.1997.6687
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发表时间：
1998-01-01
影响因子：
5.3
通讯作者：
Muramatsu, T
中科院分区：
文献类型：
--
作者：
Mochizuki, R;Takeda, A;Muramatsu, T
Midkine (MK) 是一种视黄酸响应基因产物，是一种具有神经营养活性的 13 kDa 肝素结合蛋白。先前的研究证明了 MK 在胚胎和新生儿大脑中的表达及其在体外的有效神经营养活性。然而，有关其在成熟中枢神经系统中的作用的数据仍然有限。我们通过Northern印迹、原位杂交和免疫组织化学分析检查了成年大鼠短暂前脑缺血后大脑中MK表达的变化。在对照大脑中，MK mRNA 在皮质和海马神经元中表达。缺血后，在海马 CA1 亚区发现 MK mRNA 上调，其蛋白质产物相应增加。侮辱后第4天表现出最大表达。表达 MK 的细胞分布在耗尽的 CA1 锥体细胞周围，并通过双重免疫染色鉴定为反应性星形胶质细胞。这些数据表明 MK 可能是一种损伤诱导分子，参与神经元损伤后的修复过程。 (C) 1998 年学术出版社。
Midkine (MK), a retinoic acid-responsive gene product, is a 13-kDa heparin-binding protein with neurotrophic activity. Previous studies demonstrated the expression of MK in embryonal and neonatal brains and its potent neurotrophic activities in vitro. Data concerning its role in the mature central nervous system, however, are still limited. We examined the changes of MK expression in the adult rat brain following transient forebrain ischemia, by Northern blot, in situ hybridization and immunohistochemical analyses. In the control brain, MK mRNA was expressed in the cortical and hippocampal neurons. Following the ischemia, up-regulation of MK mRNA and a corresponding increase of its protein products were found in the hippocampal CA1 subfield. The maximal expression was demonstrated on day 4 after the insult. The cells expressing MK were distributed around the depleted CA1 pyramidal cells and identified as reactive astrocytes by double immunostaining. These data suggest that MK may be an insult-induced molecule which participates in the reparative processes following neuronal injury. (C) 1998 Academic Press.