A new design of carbon fiber microelectrode for in vivo voltammetry using fused silica
A new design of carbon fiber microelectrode for in vivo voltammetry using fused silica
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DOI：
10.1016/s0165-0270(96)02207-8
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发表时间：
1997-04-25
影响因子：
3
通讯作者：
Dunn, AJ
中科院分区：
文献类型：
--
作者：
Swiergiel, AH;Palamarchouk, VS;Dunn, AJ
伏安探针由不锈钢和包覆碳纤维的熔融石英管构成。进行了电化学测试以将这些电极与市售玻璃灼烧IVEC-5电极进行比较。两种类型的电极显示出相似的下降基线和计算的稳定系数（稳定期内基线的切线）。两种电极设计对去甲肾上腺素（NE）和多巴胺（DA）的敏感性无显著差异。所有测试的电极呈线性电流响应NE和DA的浓度增加。熔融二氧化硅（FS型）电极是适合于电化学测量（在体内伏安法）和显示特性类似的市售IVEC-5玻璃密封的碳纤维微电极。在生物化学实验室中制造FS型电极很容易，不需要任何特殊设备（如微量移液器拉拔器）或玻璃处理技能。可以将另外的熔融石英管粘到电极上用于显微注射。电极非常坚固，易于操作，并且可以安装在标准立体定位仪器的臂上。电极可以做得足够长，可以到达大型动物大脑的最深处。(C)1997年Elsevier Science B.V.
Voltammetric probes were constructed from stainless steel and fused silica tubing sheathing carbon fibers. Electrochemical tests were carried out to compare these electrodes with commercially available glass-seared IVEC-5 electrodes. Electrodes of both types displayed a similar declining baseline and calculated coefficients of stabilization (tangent of baseline during a stable period). There were no significant differences in sensitivity between the two designs of electrodes to norepinephrine (NE) and dopamine (DA). All tested electrodes showed linear current responses to increasing concentrations of NE and DA. Fused silica (FS type) electrodes are suitable for electrochemical measurements (in vivo voltammetry) and display characteristics similar to those of commercially available IVEC-5 glass-sealed carbon fiber microelectrodes. Manufacture of FS type electrodes in a biochemical laboratory is easy and does not require any special equipment (such as a micropipette puller) or glass-handling skills. An additional fused silica tube can be glued to the electrode for microinjections. The electrodes are very robust, easy to handle and can be mounted on the arms of standard stereotaxic instruments. The electrodes can be made long enough to reach the deepest parts of brain of large animals. (C) 1997 Elsevier Science B.V.