Inhibition of Inflammatory Cytokines and Induction of Myeloid‐Derived Suppressor Cells by the Effects of Granulocyte and Monocyte Adsorption Apheresis
Inhibition of Inflammatory Cytokines and Induction of Myeloid‐Derived Suppressor Cells by the Effects of Granulocyte and Monocyte Adsorption Apheresis
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粒细胞和单核细胞吸附分离术对炎症细胞因子的抑制和骨髓源性抑制细胞的诱导
DOI：
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发表时间：
2017
影响因子：
1.9
通讯作者：
T. Kanekura
中科院分区：
文献类型：
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作者：
M. Sakanoue;Y. Higashi;T. Kanekura
促炎细胞因子参与了由活化的中性粒细胞和巨噬细胞引起的炎性皮肤病的发病机制。髓源性抑制细胞（MDSC）在免疫应答的调节中起着重要作用，并具有很强的免疫抑制和抗炎特性。粒细胞和单核细胞吸附单采（GMA）是一种体外单采仪器，其特征在于含有醋酸纤维素（CA）珠的柱，旨在去除致病性髓系细胞。本研究的目的是研究GMA对细胞因子产生和MDSC诱导的影响。记录GMA治疗前后血清中各种炎性细胞因子水平及外周血中MDSC的发生率。在38例患者中采用悬浮阵列法测定细胞因子。在8名患者中通过FACS分析MDSC的发生率，并使用模拟GMA的微柱系统检查GMA对体外MDSC诱导的影响。GMA能显著降低血清IL-2 R α（P = 0.030）、IL-8（P = 0.018）和MIF（P = 0.0002）水平，并能显著增加MDSCs的发生率（P = 0.030）。使用微柱系统，在5名健康志愿者的外周血中诱导MDSC;补体成分iC 3b的灭活显著抑制了体外诱导。GMA的临床有效性可能归因于通过GMA柱中的CA珠激活iC 3b抑制促炎细胞因子和诱导抗炎MDSC。
Pro‐inflammatory cytokines are involved in the pathogenesis of inflammatory skin diseases attributable to activated neutrophils and macrophages. Myeloid‐derived suppressor cells (MDSCs) play an important role in the regulation of the immune response and possess strong immunosuppressive and anti‐inflammatory properties. Granulocyte and monocyte adsorption apheresis (GMA), an extracorporeal apheresis instrument featuring columns containing cellulose acetate (CA) beads, is designed to remove pathogenic myeloid lineage cells. The purpose of this study was to investigate the effects of GMA on cytokine production and MDSC induction. The serum level of various inflammatory cytokines and the incidence of MDSCs in peripheral blood before and after GMA treatment were recorded. Cytokines were assayed with the suspension‐array method in 38 patients. The incidence of MDSCs was analyzed by FACS in eight patients and the effect of GMA on in vitro MDSC induction was examined using a mini‐column system that mimics GMA. The serum level of IL‐2Rα (P = 0.030), IL‐8 (P = 0.018), and MIF (P = 0.0002) was significantly decreased by GMA and the incidence of MDSCs was increased (P = 0.030). With the mini‐column system, MDSCs were induced in the peripheral blood of five healthy volunteers; the in vitro induction was significantly inhibited by inactivation of the complement component iC3b. The clinical effectiveness of GMA may be attributable to the inhibition of pro‐inflammatory cytokines and the induction of anti‐inflammatory MDSCs by iC3b activation via the CA beads in the GMA column.
影响因子：
20.3
作者：
Hsieh, Ching-Chuan;Chou, Hong-Shiue;Lu, Lina
通讯作者：
Lu, Lina
影响因子：
50.3
作者：
Yang, L;DeBusk, LM;Lin, PC
通讯作者：
Lin, PC