Comparative analysis of the cuticular waxes and related gene expression between 'Newhall' and 'Ganqi 3' navel orange during long-term cold storage
Comparative analysis of the cuticular waxes and related gene expression between 'Newhall' and 'Ganqi 3' navel orange during long-term cold storage
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DOI：
10.1016/j.plaphy.2021.09.032
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发表时间：
2021-09-29
影响因子：
6.5
通讯作者：
Liu, Yong
中科院分区：
文献类型：
--
作者：
Liu, Dechun;Ma, Qingling;Liu, Yong
我们从纽荷尔脐橙橙子（Citrus sinensis [L.]奥斯贝克湾Newhall，WT）。长期冷藏后，MT果实的失重率和采后腐烂率明显高于WT。为了了解潜在的机制，在4 ℃下贮藏150天期间，比较了WT和MT果实之间表皮蜡的形态、化学组成和基因表达的变化。贮藏90 d以上，MT果实的表皮蜡晶密度和大部分脂肪族蜡组分含量均显著低于WT果实。进一步的研究表明，果皮蜡的形态和化学组成的差异可能是WT和MT果实采后失重率和腐烂率差异的重要原因。值得注意的是，16个蜡质相关基因在WT和MT果实中的表达谱与相应表皮蜡质组分在冷藏过程中的变化趋势一致。这些结果表明，果皮蜡质的形态和化学组成可能受蜡质相关基因的调控，并在调节橙子采后失重和耐腐烂性方面起重要作用。
Previously, we obtained a wax-deficient mutant 'Ganqi 3' (MT) from 'Newhall' navel orange (Citrus sinensis [L.] Osbeck cv. Newhall, WT). The weight loss and postharvest decay in MT fruit were much higher than those in WT fruit after long-term cold storage. To understand the underlying mechanism, the changes in the morphology, chemical composition and gene expression of cuticular waxes between WT and MT fruit were compared during 150 days of storage at 4 degrees C. The density of epicuticular wax crystals and the contents of most of the aliphatic wax fractions in MT fruit were much lower than those in WT fruit over 90 days of storage. Further research revealed that the differences in the morphology and chemical composition of cuticular waxes might be important causes for the differences of postharvest weight loss and decay rates between WT and MT fruit. Notably, the expression profiles of 16 wax-related genes in WT and MT fruit were consistent with the change trends of corresponding cuticular wax components during cold storage. These results suggest that the morphology and chemical composition of cuticular waxes may be regulated by wax-related genes and play an important role in regulating the postharvest weight loss and the tolerances to postharvest decay in navel orange.