Skin Exposure to Narrow Band Ultraviolet (UVB) Light Modulates the Human Intestinal Microbiome
Skin Exposure to Narrow Band Ultraviolet (UVB) Light Modulates the Human Intestinal Microbiome
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DOI：
10.3389/fmicb.2019.02410
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发表时间：
2019-10-24
影响因子：
5.2
通讯作者：
Vallance, Bruce A.
中科院分区：
文献类型：
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作者：
Bosman, Else S.;Albert, Arianne Y.;Vallance, Bruce A.
最近全球范围内特发性免疫和炎症性疾病（如多发性硬化症（MS）和炎症性肠病（IBD））的增加与西方社会生活方式和环境的变化有关。这些包括减少暴露于阳光/UVB光和随后的维生素D生产受损，以及肠道微生物组成的生态失调变化。尽管它们之间存在关联，但目前尚不清楚UVB光与肠道微生物组之间是否存在任何直接联系。在这项研究中，我们研究了将皮肤暴露于窄带紫外线B（NB-UVB）光以增加血清维生素D水平是否也会调节人体肠道微生物群的组成。NB-UVB光的作用在临床初步研究中使用健康人类女性群组（n = 21）进行研究。参与者被分为在研究开始前整个冬天服用维生素D补充剂的人（VDS+）和没有服用维生素D补充剂的人（VDS-）。在同一周内三次NB-UVB照射后，参与者的血清25（OH）D水平平均增加7.3 nmol/L。血清反应与起始25-羟基维生素D [25（OH）D]血清浓度呈负相关。使用16 S rRNA测序的粪便微生物群组成分析显示，暴露于NB-UVB显著增加了VDS-组的α和β多样性，而VDS+组没有变化。根据线性判别分析（LDA）预测，在UVB暴露后，来自几个科的细菌富集在VDS-组中，包括毛螺菌科（Lachnospiracheae）、Rikenellaceae、脱硫杆菌科（Desulfobacteraceae）、瓦氏梭菌BB 60组、梭菌科XIII、Coriobacteriaceae、Marinifilaceae和瘤胃球菌。血清25（OH）D浓度显示出相关性的相对丰度的毛螺菌科，特别是成员的Lachnopsira和Fusicatenibacter属。这是第一项研究表明，低25（OH）D血清水平的人在NB-UVB皮肤暴露和25（OH）D水平增加时，肠道微生物组会发生明显变化，这表明存在一种新的皮肤-肠道轴，可用于促进肠道稳态和健康。
The recent worldwide rise in idiopathic immune and inflammatory diseases such as multiple sclerosis (MS) and inflammatory bowel diseases (IBD) has been linked to Western society-based changes in lifestyle and environment. These include decreased exposure to sunlight/UVB light and subsequent impairment in the production of vitamin D, as well as dysbiotic changes in the makeup of the gut microbiome. Despite their association, it is unclear if there are any direct links between UVB light and the gut microbiome. In this study we investigated whether exposing the skin to Narrow Band Ultraviolet B (NB-UVB) light to increase serum vitamin D levels would also modulate the makeup of the human intestinal microbiota. The effects of NB-UVB light were studied in a clinical pilot study using a healthy human female cohort (n = 21). Participants were divided into those that took vitamin D supplements throughout the winter prior to the start of the study (VDS+) and those who did not (VDS-). After three NB-UVB light exposures within the same week, the serum 25(OH)D levels of participants increased on average 7.3 nmol/L. The serum response was negatively correlated to the starting 25-hydroxy vitamin D [25(OH)D] serum concentration. Fecal microbiota composition analysis using 16S rRNA sequencing showed that exposure to NB-UVB significantly increased alpha and beta diversity in the VDS- group whereas there were no changes in the VDS+ group. Bacteria from several families were enriched in the VDS- group after the UVB exposures according to a Linear Discriminant Analysis (LDA) prediction, including Lachnospiracheae, Rikenellaceae, Desulfobacteraceae, Clostridiales vadinBB60 group, Clostridia Family XIII, Coriobacteriaceae, Marinifilaceae, and Ruminococcus. The serum 25(OH)D concentrations showed a correlation with the relative abundance of the Lachnospiraceae, specifically members of the Lachnopsira and Fusicatenibacter genera. This is the first study to show that humans with low 25(OH)D serum levels display overt changes in their intestinal microbiome in response to NB-UVB skin exposure and increases in 25(OH)D levels, suggesting the existence of a novel skin-gut axis that could be used to promote intestinal homeostasis and health.