The metal transporter ZIP13 supplies iron into the secretory pathway in Drosophila melanogaster.
The metal transporter ZIP13 supplies iron into the secretory pathway in Drosophila melanogaster.
复制标题
金属转运蛋白 ZIP13 将铁供应到果蝇的分泌途径中
作者：
Xiao G;Wan Z;Fan Q;Tang X;Zhou B
细胞内的铁转移过程还不清楚，负责将铁输送到分泌室的铁转运体的身份仍然难以捉摸。在这项研究中，我们展示了果蝇ZIP13(Slc39a13)，一个可能的锌进口者，完成了铁的流出作用。干扰dZIP13的表达可导致铁可挽救的铁吸收缺陷、胞浆中铁的增加和分泌间隔室的减少、铁蛋白铁负荷的失败和胶原的异常分泌。DZIP13在大肠杆菌中的表达与宿主铁依赖生长和铁抗性有关。重要的是，使用铁同位素的时间序列运输分析表明，dZIP13具有很强的铁出口活性。对dZIP13作为铁转运体的鉴定表明，hZIP13缺陷的Ehler-Danlos综合征的脊柱发育不良形式可能是由于铁向分泌室输送失败所致。我们的结果也扩大了我们对铁代谢紊乱缺陷范围的认识。DOI：http://dx.doi.org/10.7554/eLife.03191.001
The intracellular iron transfer process is not well understood, and the identity of the iron transporter responsible for iron delivery to the secretory compartments remains elusive. In this study, we show Drosophila ZIP13 (Slc39a13), a presumed zinc importer, fulfills the iron effluxing role. Interfering with dZIP13 expression causes iron-rescuable iron absorption defect, simultaneous iron increase in the cytosol and decrease in the secretory compartments, failure of ferritin iron loading, and abnormal collagen secretion. dZIP13 expression in E. coli confers upon the host iron-dependent growth and iron resistance. Importantly, time-coursed transport assays using an iron isotope indicated a potent iron exporting activity of dZIP13. The identification of dZIP13 as an iron transporter suggests that the spondylocheiro dysplastic form of Ehlers–Danlos syndrome, in which hZIP13 is defective, is likely due to a failure of iron delivery to the secretory compartments. Our results also broaden our knowledge of the scope of defects from iron dyshomeostasis. DOI: http://dx.doi.org/10.7554/eLife.03191.001