Vascular endothelial growth factor (vascular permeability factor) expression in injured rat brain
Vascular endothelial growth factor (vascular permeability factor) expression in injured rat brain
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DOI：
10.1002/(sici)1097-4547(19970815)49:4
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发表时间：
1997-08
影响因子：
4.2
通讯作者：
E. Papavassiliou;N. Gogate;M. Proescholdt;John D Heiss;S. Walbridge;N. Edwards;E. Oldfield;M. Merrill
中科院分区：
文献类型：
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作者：
E. Papavassiliou;N. Gogate;M. Proescholdt;John D Heiss;S. Walbridge;N. Edwards;E. Oldfield;M. Merrill
本研究观察了大鼠针刺性和冷冻性脑损伤模型中血管内皮生长因子（VEGF）和血管通透性因子（VPF）的表达。抗VEGF抗体的免疫组织化学染色显示两处病变内和周围的VEGF阳性细胞增加。在形态学上，损伤诱导的VEGF阳性细胞类似于星形胶质细胞。星形胶质细胞标志物胶质细胞酸性蛋白（GFAP）和VEGF的双重免疫荧光染色直接证明了对这些损伤做出反应的VEGF阳性细胞是星形胶质细胞。VEGF在星形胶质细胞中的表达在损伤后第3天和第4天在细胞数量和受影响面积方面达到最大。VEGF阳性细胞的增加在冷冻损伤中比在刺伤中更广泛，并且发生在损伤和非损伤的半球中。两种损伤后3周，VEGF阳性细胞仍然存在，但它们的数量减少，并且它们的分布仅限于病变附近。这些观察结果表明，星形胶质细胞通过增加VEGF表达对损伤作出反应，表明VEGF可能参与中枢神经系统对损伤的反应。J. Neurosci. Res. 49：451-460，1997.© 1997 Wiley利斯公司
We investigated the expression of vascular endothelial growth factor (VEGF)/vascular permeability factor (VPF) in stab and freeze brain injury models in rats. Immunohistochemical staining with anti‐VEGF antibodies demonstrated an increase in VEGF‐positive cells in and around both lesions. Morphologically, the injury‐induced VEGF‐positive cells resembled astrocytes. Double immunofluorescent staining for the astrocytic marker glial fibrillary acidic protein (GFAP) and VEGF demonstrated directly that VEGF‐positive cells which appeared in response to these injuries were astrocytes. VEGF expression in astrocytes was maximal on days 3 and 4 after injury in terms of both cell number and affected area. The increase in VEGF‐positive cells was more widespread in the freeze lesion than in the stab wound, and occurred in both the lesioned and nonlesioned hemispheres. VEGF‐positive cells were still present 3 weeks after both injuries, but their numbers were reduced and their distribution became limited to the immediate vicinity of the lesions. These observations indicate that astrocytes react to injury by increasing VEGF expression, suggesting that VEGF might participate in the central nervous system response to injury. J. Neurosci. Res. 49:451–460, 1997. © 1997 Wiley‐Liss, Inc.