Description of Pandoraea gen. nov with Pandoraea apista sp nov., Pandoraea pulmonicola sp nov., Pandoraea pnomenusa sp nov., Pandoraea sputorum sp nov and Pandoraea norimbergensis comb. nov.
Description of Pandoraea gen. nov with Pandoraea apista sp nov., Pandoraea pulmonicola sp nov., Pandoraea pnomenusa sp nov., Pandoraea sputorum sp nov and Pandoraea norimbergensis comb. nov.
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DOI：
10.1099/00207713-50-2-887
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发表时间：
2000-03-01
影响因子：
2.8
通讯作者：
Vandamme, P
中科院分区：
文献类型：
--
作者：
Coenye, T;Falsen, E;Vandamme, P
对一组分离株进行了多相分类学研究，初步鉴定为洋葱伯克霍尔德菌（Burkholderia cepacia）、皮氏罗尔斯通氏菌（Ralstonia pickettii）或少孢罗尔斯通氏菌（Ralstonia paucula）（原CDC Ⅳ c-2群）。这些分离株主要从囊性纤维化患者的痰液或土壤中培养。16 S rDNA序列分析表明，这些菌株属于变形菌门β亚类，与伯克霍尔德菌属和罗尔斯通菌属关系最近。根据基因型和表型特征，将这些菌株划分为一个新属--潘多拉菌属。新属的DNA碱基组成在61.2 ~ 64.3mol%之间。该新属包括4个新种，蜜蜂潘多拉属Pandoraea apista（模式种）（模式菌株为LMG 16407（T））、肺潘多拉菌（Pandoraea pulmonicola（模式菌株为LMG 18106（T））、Pandoraea pnomenusa（模式菌株为LMG 18087（T））和Pandoraea acutorum（模式菌株是LMG 18819（T）），和Pandoraea norimbergensis（Wittke等人，1997）comb. nov，（模式菌株是LMG 18379（T））。可用的临床数据表明，这些生物体中的至少一些可能在囊性纤维化患者中引起慢性感染，并且可以在囊性纤维化患者中传播。
A polyphasic taxonomic study was performed on a group of isolates tentatively identified as Burkholderia cepacia, Ralstonia pickettii or Ralstonia paucula (formerly known as CDC group IVc-2), The isolates were mainly cultured from sputum of cystic fibrosis patients or from soil, SDS-PAGE of whole-cell proteins and AFLP fingerprinting distinguished at least five different species, and this was confirmed by DNA-DNA hybridizations. 16S rDNA sequence analysis of representative strains indicated that these organisms belong to the beta-subclass of the Proteobacteria, with the genera Burkholderia and Ralstonia as closest neighbours, Based on genotypic and phenotypic characteristics, the organisms were classified in a novel genus, Pandoraea. The DNA base composition of the members of the new genus is between 61.2 and 64.3 mol%, This novel genus includes four new species, Pandoraea apista (the type species) (type strain is LMG 16407(T)), Pandoraea pulmonicola (type strain is LMG 18106(T)), Pandoraea pnomenusa (type strain is LMG 18087(T)) and Pandoraea sputorum (type strain is LMG 18819(T)), and Pandoraea norimbergensis (Wittke et al, 1997) comb. nov, (type strain is LMG 18379(T)), The available clinical data indicate that at least some of these organisms may cause chronic infection in, and can be transmitted amongst, cystic fibrosis patients.