In vivo and in vitro interferon induction in chickens by S-28828, an imidazoquinolinamine immunoenhancer
In vivo and in vitro interferon induction in chickens by S-28828, an imidazoquinolinamine immunoenhancer
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DOI：
10.1089/jir.1996.16.327
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发表时间：
1996-04-01
影响因子：
2.3
通讯作者：
Miller, RL
中科院分区：
文献类型：
--
作者：
Karaca, K;Sharma, JM;Miller, RL
咪喹莫特及其类似物属于咪唑喹啉胺类，通过细胞因子诱导激活免疫系统，在哺乳动物中具有抗肿瘤和抗病毒作用。在这项研究中，我们发现了一种相关的类似物，命名为s -28828，在体内、离体和体外诱导鸡体内的干扰素（IFN）和巨噬细胞激活因子（MAF）。经口服给药后，在鸡血清中检测到IFN和MAF。治疗后2 h血清IFN水平最高。虽然在治疗后8、24和48小时，鸡的血清中没有检测到IFN，但在这些时间点，干扰素诱导酶2’-5’低聚腺苷酸合成酶（2’5’OAS）水平较高。体外和离体研究表明，脾细胞、骨髓细胞（BM）和外周血白细胞（PBL）均能产生IFN和MAF，但脾细胞的产生量最高。结果表明，S-28828可能是一种有效的鸡免疫增强剂和抗病毒药物。
Imiquimod and its analogs belonging to a class of imidazoquinolinamines, activate immune system via cytokine induction, and have antitumor and antiviral effects in mammals. In this study, we showed that a related analog, designated S-28828, induced interferon (IFN) and macrophage activating cytokine(s) (macrophage activating factor, MAF) in chickens in vivo, ex vivo, and in vitro. IFN and MAF were detectable in the serum of chickens following oral administration. Serum IFN levels were the highest at 2 h after treatment. Although there was no detectable IFN in sera of chickens at 8, 24, and 48 h after treatment, high levels of interferon inducible enzyme, 2'-5' oligoadenylate synthase (2'5' OAS) were present at these time points. In vitro and ex vivo studies showed that spleen cells, bone marrow (BM) cells, and peripheral blood leukocytes (PBL) were capable of producing IFN and MAF, although spleen cells produced the highest levels. Our results suggest that S-28828 administered orally may be a useful immunoenhancing and antiviral agent for chickens.