Global transcriptional programs reveal a carbon source foraging strategy by Escherichia coli
Global transcriptional programs reveal a carbon source foraging strategy by Escherichia coli
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DOI：
10.1074/jbc.m414050200
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发表时间：
2005-04-22
影响因子：
4.8
通讯作者：
Blattner, FR
中科院分区：
文献类型：
--
作者：
Liu, MZ;Durfee, T;Blattner, FR
通过探索在六种不同碳源上生长的大肠杆菌的全局基因表达，我们发现了一个惊人的基因组转录模式：随着碳底物质量的下降，细胞系统地增加表达的基因数量。基因诱导以分级方式发生，包括许多因素，用于吸收和代谢更好但目前不可用的碳源。同时，细胞也增加了它们的运动性。因此，随着环境的生长潜力下降，细胞似乎会逐渐将更多的能量投入到改善环境的可能性上。这种适应并不是传统监管模式所能预测的。我们还观察到基因激活和rRNA合成之间的负相关性，这表明重新分配RNA聚合酶（RNAP）有助于扩大基因组激活。RNAP在体内分布的显着差异，使用RNAP-绿色荧光蛋白融合监测，从能源丰富和能源贫乏的碳源文化支持这一假设。总之，这些发现代表了基质特异性和全球监管系统的整合，并可能是一个细菌近似后生动物的风险倾向觅食行为。
By exploring global gene expression of Escherichia coli growing on six different carbon sources, we discovered a striking genome transcription pattern: as carbon substrate quality declines, cells systematically increase the number of genes expressed. Gene induction occurs in a hierarchical manner and includes many factors for uptake and metabolism of better but currently unavailable carbon sources. Concomitantly, cells also increase their motility. Thus, as the growth potential of the environment decreases, cells appear to devote progressively more energy on the mere possibility of improving conditions. This adaptation is not what would be predicated by classic regulatory models alone. We also observe an inverse correlation between gene activation and rRNA synthesis suggesting that reapportioning RNA polymerase ( RNAP) contributes to the expanded genome activation. Significant differences in RNAP distribution in vivo, monitored using an RNAP-green fluorescent protein fusion, from energy-rich and energy-poor carbon source cultures support this hypothesis. Together, these findings represent the integration of both substrate-specific and global regulatory systems, and may be a bacterial approximation to metazoan risk-prone foraging behavior.