Quantitative flow cytometric analysis of ABO red cell antigens.
Quantitative flow cytometric analysis of ABO red cell antigens.
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ABO 红细胞抗原的定量流式细胞术分析。
DOI：
10.1002/cyto.990120611
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
E. Fibach
中科院分区：
文献类型：
--
作者：
R. Sharon;E. Fibach
应用流式细胞术对不同红细胞A、B、H抗原的表达进行了定量比较。用荧光素偶联的抗H凝集素直接标记H物质，用单克隆抗A或抗B抗体间接染色A和B抗原，然后用荧光素（FITC）或藻红蛋白（PE）标记的抗小鼠免疫球蛋白（IG）抗体进行荧光染色。在每个样品中发现细胞荧光强度的十倍以上的差异。测定每种抗原阳性亚群的百分比和平均荧光。每个RBC群体的A、B或H抗原表达通过复合平均值表征，复合平均值是这两个参数的计算乘积。结果表明，A或B的复合平均值与H之间存在倒数关系。发现A/H或B/H的比率提供的信息最多。A或B的纯合子的比率分别大于200和大于30，而杂合子（AO或BO）的比率小于5。这种方法也可以区分A1和A2;携带A1表型的红细胞（通过与抗A1凝集素凝集测定）显示出比携带A2的红细胞更高的A/H比。与在从不同个体获得的RBC中发现的A（或B）和H的表达的相互作用相反，在给定群体内的个体细胞的这些抗原的表达中发现了直接相关性。（250字处删节）
A flow cytometry method has been employed to quantitatively compare the expression of A, B and H antigens on various red blood cells (RBC). The H substance was directly labelled by fluorescein-conjugated anti-H lectin and the A and B antigens by indirect staining first with monoclonal anti-A or anti-B antibodies followed by fluorescently, fluorescein (FITC) or phycoerythrin (PE), labelled anti-mouse immunoglobulin (Ig) antibodies. More than a ten-fold difference in cellular fluorescence intensity was found within each sample. Both the percentage and the mean fluorescence of the positive subpopulation for each antigen were determined. Each RBC population was characterized with respect to the expression of A, B or H antigen by a compound mean value that was the calculated product of these two parameters. The results demonstrated a reciprocal relationship between the compound means of A or B and H. The ratio of A/H or B/H was found to be most informative. Homozygotes for A or B had ratios of greater than 200 and greater than 30, respectively, while heterozygotes (AO or BO) had ratios of less than 5. This method could also distinguish between A1 and A2; RBC carrying the A1 phenotype (as determined by agglutination with anti-A1 lectin) showed a higher A/H ratio than those carrying A2. In contrast to the reciprocity in the expression of A (or B) and H found in RBC obtained from different individuals, a direct correlation was found in the expression of these antigens by individual cells within a given population.(ABSTRACT TRUNCATED AT 250 WORDS)