Antisense sequence-directed cross-linking of DNA oligonucleotides by mitomycin C.
Antisense sequence-directed cross-linking of DNA oligonucleotides by mitomycin C.
复制标题
丝裂霉素 C 进行 DNA 寡核苷酸反义序列定向交联。
DOI：
10.1021/bc960054r
复制
发表时间：
1996
期刊：
影响因子：
--
通讯作者：
Tomasz,M
中科院分区：
文献类型：
--
作者：
Maruenda,H;Tomasz,M
Oligodeoxyribonucleotides (ODNs) conjugated with mitomycin C (MC) via (−CH2−)ntethers of different lengths (n= 6, 12) to their terminal 5‘-phosphate were synthesized, and their interaction with target complementary single-stranded DNA oligonucleotides was investigated. MC, a clinically used natural anticancer drug, is known to act as a bioreductive alkylating agent of duplex DNA with a remarkable preference for 5‘-d(CG) sequences. The usual enzymatic bioreductive techniques known to trigger MC to alkylate DNA were employed in the reaction between the MC−oligonucleotide conjugates and their targets radiolabeled by32P at their 5‘-phosphate. A slow-moving radiolabeled product, detected by polyacrylamide gel electrophoresis using phosphorimaging techniques, was obtained in 15−25% yield with complementary DNA as target. Formation of these products was dependent upon complementary duplex formation. Evidence is presented that the DNA target is alkylated by the mitomycin C moiety of the ODN conjugate at the 2-amino group of a guanine base. These findings suggest that the MC−ODN conjugates may be useful specific inhibitors of cellular or viral gene expression. To our knowledge this is the first report on ODN conjugates of a reductively activated drug of known therapeutic value.
