Stability of neural differentiation in human adipose derived stem cells by two induction protocols
Stability of neural differentiation in human adipose derived stem cells by two induction protocols
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DOI：
10.1016/j.tice.2011.11.006
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发表时间：
2012-04-01
期刊：
影响因子：
2.6
通讯作者：
Oryan, Shahrbanoo
中科院分区：
文献类型：
--
作者：
Ahmadi, Nafiseh;Razavi, Shahnaz;Oryan, Shahrbanoo
有证据表明，脂肪干细胞（ADSCs）可以向神经细胞方向分化。脂肪干细胞可以在体外快速扩增，并且可以通过侵入性较小的方法获得。在本研究中，我们尝试比较两种诱导方法对人脂肪干细胞神经分化的稳定性。对于方案1，通过化学诱导来诱导ADSC。在方案2中，处理ADSC以形成球体。然后，将单个细胞培养在添加特殊成分的神经基础培养基中。用免疫细胞化学和半定量RT-PCR技术检测分化的ADSCs中Nestin、MAP 2和GFAP的表达。MTT法检测细胞活力和增殖情况，免疫细胞化学分析显示，两种方法培养的ADSCs均表达大量神经特异性标志物。在RT-PCR中，方案1显示MAP 2表达的最高百分比，但随着时间的推移，神经样状态是可逆的。方案2在第1周发现Nestin的表达，而MAP 2和GFAP的表达在3周后增加。比较分析表明，化学诱导法诱导的ADSCs向神经样细胞分化速度快，但时间较短，而神经球形成法诱导的ADSCs向神经样细胞分化速度较稳定。(C)2011爱思唯尔有限公司保留所有权利。
There are some evidences for suggesting that adipose derived stem cells (ADSCs) can be differentiated to the fate of neural cell type. ADSCs can be expanded rapidly in vitro and can be obtained by a less invasive method. In this study, we attempted to compare the stability of neural differentiation in human ADSCs by using two induction protocols.Isolated ADSCs were induced into neural-like cells using diverse effects of two specific procedures. For protocol 1, ADSCs were induced by chemical induction. In protocol 2, ADSCs were treated for sphere formation. Then, the singled cells were cultured in neurobasal media supplemented with special components. Differentiated ADSCs were evaluated for Nestin, MAP2 and GFAP expression by immunocytochemistry and semi quantitative RT-PCR techniques. Moreover, MTT assay was employed to detect cell viability and proliferation.Immunocytochemical analysis of both protocols demonstrated that ADSCs had large expression of the neural-specific markers. In RT-PCR, protocol 1 showed the highest percentage of MAP2 expression, but with time passing, the neural like state was reversible. Protocol 2 found with express of Nestin at week 1, however MAP2 and GFAP expression increased after 3 weeks. The neural-like cells produced by protocol 1 led to the further cell death.Comparative analysis showed that neural-like cell differentiation of ADSCs in chemical induction protocol was rapid but transitory, while it was approximately steady in neurosphere formation protocol. (C) 2011 Elsevier Ltd. All rights reserved.