Source of dietary sucrose influences development of leptin resistance in male and female rats
Source of dietary sucrose influences development of leptin resistance in male and female rats
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DOI：
10.1152/ajpregu.00384.2017
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发表时间：
2018-04-01
影响因子：
2.8
通讯作者：
Harris, Ruth B. S.
中科院分区：
文献类型：
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作者：
Harris, Ruth B. S.
雄性大鼠除了食物之外还提供30%的蔗糖溶液，在不增加能量摄入或体脂的情况下产生瘦素抵抗。本研究测试了瘦素抵抗是否依赖于蔗糖的物理形式。给Sprague-Dawley大鼠提供无蔗糖（NS）饮食、66.6%能量作为蔗糖（HS）饮食或NS饮食+30%蔗糖溶液（LS）。LS大鼠的蔗糖摄入量与HS大鼠相当，但总碳水化合物摄入量超过LIS大鼠。33天后，雄性和雌性LS大鼠对外周给药的瘦素对食物摄入的抑制作用具有抗性。LS大鼠在光照和黑暗期间饮用少量频繁的蔗糖餐，而HS大鼠在黑暗期间比光照期间消耗更多的餐，并对瘦素保持反应。饮食不影响每日能量摄入或胰岛素敏感性。雌性大鼠的体脂略有增加。在雄性LS大鼠中，戒断蔗糖5天后，瘦素敏感性恢复。这种快速逆转表明，瘦素抵抗与饮用蔗糖的代谢影响有关。进行了一项实验以测试LS大鼠中己糖胺生物合成途径的活性和瘦素信号蛋白的糖化是否增加，但结果不确定。最后一项实验确定，雌性LS大鼠在18天内获得蔗糖溶液的瘦素抵抗，并且体脂肪的小但显著的增加与脂肪细胞葡萄糖利用和胰岛素反应性的增加相关，这可能是继发于脂肪细胞瘦素抵抗。
Male rats offered 30% sucrose solution in addition to chow develop leptin resistance without an increase in energy intake or body fat. This study tested whether the leptin resistance was dependent on the physical form of the sucrose. Sprague-Dawley rats were offered a sucrose-free (NS) diet, a 66.6% of energy as sucrose (HS) diet, or the NS diet + 30% sucrose solution (LS). Sucrose intake of LS rats equaled that of HS rats, but total carbohydrate intake exceeded that of LIS rats. After 33 days, male and female LS rats were resistant to the inhibitory effect of peripherally administered leptin on food intake. LS rats drank small, frequent meals of sucrose during light and dark periods, whereas HS rats consumed more meals during the dark than the light period and remained responsive to leptin. Diet did not affect daily energy intake or insulin sensitivity. There was a small increase in body fat in the female rats. Leptin sensitivity was restored within 5 days of withdrawal from sucrose in male LS rats. This rapid reversal suggested that leptin resistance was associated with the metabolic impact of drinking sucrose. An experiment was carried out to test whether activity of the hexosamine biosynthetic pathway and glycation of leptin signaling proteins were increased in LS rats, but the results were equivocal. A final experiment determined that female LS rats were leptin-resistant within 18 days of access to sucrose solution and that the small, but significant, increase in body fat was associated with increased adipocyte glucose utilization and insulin responsiveness, which may have been secondary to adipocyte leptin resistance.