CELLULAR LOCATION OF A TREPONEMA-DENTICOLA CHYMOTRYPSINLIKE PROTEASE AND IMPORTANCE OF THE PROTEASE IN MIGRATION THROUGH THE BASEMENT-MEMBRANE
CELLULAR LOCATION OF A TREPONEMA-DENTICOLA CHYMOTRYPSINLIKE PROTEASE AND IMPORTANCE OF THE PROTEASE IN MIGRATION THROUGH THE BASEMENT-MEMBRANE
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DOI：
10.1128/iai.58.2.347-351.1990
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发表时间：
1990-02-01
影响因子：
3.1
通讯作者：
MCBRIDE, BC
中科院分区：
文献类型：
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作者：
GRENIER, D;UITTO, VJ;MCBRIDE, BC
使用多种免疫学方法来定位细胞相关的密螺旋体糜蛋白酶样蛋白酶。间接免疫荧光染色、免疫金标记和酶联免疫吸附测定均表明蛋白酶附着在细胞膜的外部。通过跟踪重构基底膜材料（Matrigel）的降解和螺旋体从凝胶中的释放来评估 T. denticola 的侵袭能力。在胰凝乳蛋白酶样活性增加的条件下，更多的螺旋体从凝胶中迁移出来。蛋白酶抑制剂大大减少了移出凝胶的细胞数量。纯化的胰凝乳蛋白酶样蛋白酶降解基底膜成分IV型胶原、层粘连蛋白和纤连蛋白。研究提示T. denticola胰凝乳蛋白酶样蛋白酶可能在基底膜的侵袭和破坏中发挥重要作用。
A number of immunological methods were used to localize a cell-associated Treponema denticola chymotrypsinlike protease. Indirect immunofluorescence staining, immunogold labeling, and an enzyme-linked immunosorbent assay all indicated that the protease was attached to the outside of the cell envelope. The invasive capability of T. denticola was evaluated by following the degradation of a reconstituted basement membrane material (Matrigel) and the release of spirochetes from the gel. Under conditions where the chymotrypsinlike activity was increased, more spirochetes migrated from the gel. Protease inhibitors strongly reduced the number of cells that moved out of the gel. The purified chymotrypsinlike protease degraded the basement membrane components type IV collagen, laminin, and fibrinonectin. The study suggests that the T. denticola chymotrypsinlike protease may play an important role in the invasion and destruction of basement membrane.