Genome-wide identification and comparative analysis of resistance genes in Brassica juncea
Genome-wide identification and comparative analysis of resistance genes in Brassica juncea
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DOI：
10.1007/s11032-020-01159-z
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发表时间：
2020-08-03
影响因子：
3.1
通讯作者：
Batley, Jacqueline
中科院分区：
文献类型：
--
作者：
Inturrisi, Fabian;Bayer, Philipp E.;Batley, Jacqueline
甘蓝型油菜已显示出对几种疾病的抗性，包括黑腿病、白锈病、下胚轴腐病、萝卜花叶病毒、叶枯病、黑腐病和稻瘟病。抗病基因的鉴定对培育抗病优良品种具有重要意义。最大的耐药基因家族是核苷酸结合位点富亮氨酸重复序列基因（NLRs），它分为两个主要亚家族：toll/白细胞介素-1受体nlr （TNL）和卷曲卷曲nlr （CNL）蛋白。在这里，我们提出了NLR基因家族在b的基因组组织和进化历史的第一个研究。与二倍体祖先的比较分析。rapaandB。黑质。在b中共鉴定出289个NLR基因。TNL基因与非TNL基因的比例为0.61:1。NLR基因的分布是随机且不均匀的，A04染色体不含NLR基因。区域结构分析显示，24%的NLR基因是典型的耐药基因，包含了三个结构域（TIR/CC、NBS、LRR），其余基因部分缺失或截断。共有45%的NLR基因被发现在b中物理聚集。junceagenome。用OrthoFinder对NLR基因进行分析，发现大多数物理簇（63%）在b。juncea来自同一个正族。该研究为油菜新品种NLR候选基因的鉴定和鉴定提供了有价值的资源。
Disease resistance inBrassica junceahas been shown against several diseases including blackleg, white rust, hypocotyl rot, turnip mosaic virus, leaf blight, black rot, and Alternaria blight. The identification of disease resistance genes is important to develop elite cultivars, which can survive exposure to these diseases. The largest family of resistance genes are nucleotide-binding site leucine-rich repeat genes (NLRs) which are classified into two major subfamilies: toll/interleukin-1 receptor-NLR (TNL) and coiled-coil-NLR (CNL) proteins. Here we present the first study of the genomic organization and evolutionary history of the NLR gene family inB. juncea, with comparative analysis to the diploid progenitorsB. rapaandB. nigra. A total of 289 NLR genes were identified inB. juncea, with a ratio of 0.61:1 of TNL to non-TNL genes. The distribution of NLR genes was random and uneven, with chromosome A04 containing no NLR genes. Domain structure analysis revealed that 24% of NLR genes are typical resistance genes containing all three domains (TIR/CC, NBS, LRR), whereas the remaining genes are partially deleted or truncated. A total of 45% NLR genes were found to be physically clustered in theB. junceagenome. The NLR genes were analysed with OrthoFinder which showed that most physical clusters (63%) inB. junceacame from the same orthogroup. This study provides a valuable resource for the identification and characterization of candidate NLR genes for Brassica crop improvement.