Pertussis toxin-sensitive pathway in the stimulation of c-myc expression and DNA synthesis by bombesin.
Pertussis toxin-sensitive pathway in the stimulation of c-myc expression and DNA synthesis by bombesin.
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铃蟾肽刺激 c-myc 表达和 DNA 合成的百日咳毒素敏感途径。
DOI：
10.1126/science.3465038
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Williams,LT
中科院分区：
文献类型：
--
作者：
Letterio,JJ;Coughlin,SR;Williams,LT
铃蟾肽样肽是瑞士 3T3 成纤维细胞、人支气管上皮细胞和从小细胞肺癌中分离出的细胞的有效有丝分裂原。通过研究百日咳博德特氏菌毒素对铃蟾肽刺激的有丝分裂的影响，研究了铃蟾肽增殖反应中的信号转导机制。在纳摩尔浓度下，铃蟾肽可提高 Swiss 3T3 细胞中 c-mycmessenger RNA 的水平并刺激 DNA 合成。用百日咳毒素（5 纳克/毫升）处理细胞完全阻断铃蟾肽增强的 c-myc 表达，并消除铃蟾肽刺激的 DNA 合成。这种治疗对血小板衍生生长因子或佛波醇 12,13-二丁酸酯的促有丝分裂反应基本上没有影响。这些结果表明，铃蟾肽样生长因子的促有丝分裂作用是通过百日咳毒素敏感的鸟嘌呤核苷酸结合蛋白介导的。此外，他们表明铃蟾肽样生长因子通过与血小板衍生生长因子激活的途径不同的途径发挥作用。
The bombesin-like peptides are potent mitogens for Swiss 3T3 fibroblasts, human bronchial epithelial cells, and cells isolated from small cell carcinoma of the lung. The mechanism of signal transduction in the proliferative response to bombesin was investigated by studying the effect ofBordetella pertussistoxin on bombesin-stimulated mitogenesis. At nanomolar concentrations, bombesin increased levels of c-mycmessenger RNA and stimulated DNA synthesis in Swiss 3T3 cells. Treatment of the cells with pertussis toxin (5 nanograms per milliliter) completely blocked bombesin-enhanced c-mycexpression and eliminated bombesin-stimulated DNA synthesis. This treatment had essentially no effect on the mitogenic responses to either platelet-derived growth factor or phorbol 12,13-dibutyrate. These results suggest that the mitogenic actions of bombesin-like growth factors are mediated through a pertussis toxin-sensitive guanine nucleotide-binding protein. Furthermore they indicate that bombesin-like growth factors act through pathways that are different from those activated by platelet-derived growth factor.