STRUCTURAL AND FUNCTIONAL-RELATIONSHIPS BETWEEN PROKARYOTIC AND EUKARYOTIC DNA-POLYMERASES
STRUCTURAL AND FUNCTIONAL-RELATIONSHIPS BETWEEN PROKARYOTIC AND EUKARYOTIC DNA-POLYMERASES
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DOI：
10.1002/j.1460-2075.1987.tb02770.x
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发表时间：
1987-12-01
期刊：
影响因子：
11.4
通讯作者：
BLANCO, L
中科院分区：
文献类型：
--
作者：
BERNAD, A;ZABALLOS, A;BLANCO, L
参与蛋白质引发的病毒DNA复制的枯草芽孢杆菌噬菌体v φ 29 DNA聚合酶被膦酰基乙酸（PAA）抑制，膦酰基乙酸是一种已知的α-磷酸化酶抑制剂。就像DNA聚合酶一样，通过降低延伸率。三个高度保守的氨基酸同源性区域，发现于几种病毒α-在T4 DNA聚合酶中也发现了类似于DNA聚合酶和在v φ 29 DNA聚合酶中的两种酶，其中一种被认为是PAA结合位点。这种原核酶对药物阿非迪霉素和核苷酸类似物丁基苯胺基dATP（BuAdATP）和丁基苯基dGTP（BuPdGTP）高度敏感，已知它们是真核α-谷氨酰胺的特异性抑制剂。比如DNA聚合酶已经鉴定了来自酵母线性质粒pGKL 1和来自玉米的S1线粒体DNA的两种潜在的DNA聚合酶，这是基于它们含有三个氨基酸同源性保守区的事实。来自原核和真核来源的DNA聚合酶的比较显示，除了高度保守的结构域之外，还具有广泛的氨基酸同源性。这些发现反映了假设无关的DNA聚合酶之间的进化关系。
The Bacillus subtilis phage .vphi.29 DNA polymerase, involved in protein-primed viral DNA replication, was inhibited by phosphonoacetic acid (PAA), a known inhibitor of .alpha.-like DNA polymerases, by decreasing the rate of elongation. Three highly conserved regions of amino acid homology, found in several viral .alpha.-like DNA polymerases and in the .vphi.29 DNA polymerase, one of them proposed to be the PAA binding site, were also found in the T4 DNA polymerase. This prokaryotic enzyme was highly sensitive to the drugs aphidicolin and the nucleotide analogues butylanilino dATP (BuAdATP) and butylphenyl dGTP (BuPdGTP), known to be specific inhibitors of eukaryotic .alpha.-like DNA polymerases. Two potential DNA polymerases from the linear plasmid pGKL1 from yeast and the S1 mitochondrial DNA from maize have been identified, based on the fact that they contain the three conserved regions of amino acid homology. Comarison of DNA polymerases from prokaryotic and eukaryotic origin showed extensive amino acid homology in addition to highly conserved domains. These findings reflect evolutionary relationships between hypothetically unrelated DNA polymerases.