Characterization of mixed lymphocyte reaction blocking antibodies (MLR-Bf) in human pregnancy.
Characterization of mixed lymphocyte reaction blocking antibodies (MLR-Bf) in human pregnancy.
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DOI：
10.1186/1471-2393-3-2
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发表时间：
2003-02-19
影响因子：
3.1
通讯作者：
Agrawal S
中科院分区：
文献类型：
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作者：
Pandey MK;Saxena V;Agrawal S
众所周知，在正常妊娠期间和免疫治疗阻断抗体产生后，这些抗体会抑制混合淋巴细胞反应，并且本质上也具有抗有丝分裂作用。混合淋巴细胞反应阻断抗体对丈夫的淋巴细胞具有特异性。在本研究中，试图表征正常妊娠和免疫治疗后反复自然流产的妇女中混合淋巴细胞反应阻断抗体的特征。血清取自不同妊娠期的妇女（正常妊娠的第一、第二、第三三个月和分娩后期）、免疫前后的复发性自然流产者。筛选健康（男性和女性）对照是否存在混合淋巴细胞反应阻断抗体。采用标准混合淋巴细胞反应技术评价血清对混合淋巴细胞反应的抑制作用。测试每种血清的细胞毒性抗体。根据标准方案分离免疫球蛋白G及其同种型。在本研究中，我们观察到，当将来自妊娠不同三个月的免疫球蛋白G添加到单向混合淋巴细胞反应或植物血凝素激活的淋巴细胞增殖测定中时，增殖反应受到显着抑制。当使用从反复自然流产的充分免疫妇女中分离出的免疫球蛋白 G 时，也出现了类似的情况。进一步证实，在免疫球蛋白G的所有同种型中，只有免疫球蛋白G-3被发现具有抑制作用阳性。目前的研究表明，混合淋巴细胞反应阻断抗体本质上是免疫球蛋白G-3。它是在怀孕期间以及在随后成功怀孕的反复自然流产的妇女的免疫治疗后出现的。
It is known that during normal pregnancy and after immunotherapy blocking antibodies are developed, these antibodies inhibit mixed lymphocyte reaction and are also anti-mitogenic in nature. Mixed lymphocyte reaction blocking antibodies are specific to the husband's lymphocytes. In the present study an attempt has been made to characterize the mixed lymphocyte reaction blocking antibodies in normal pregnancy and in women with recurrent spontaneous abortion after immunotherapy. Serum was obtained from women of different gestational windows of pregnancy (Ist, IInd, IIIrd trimesters and post delivery period of normal pregnancy), recurrent spontaneous aborters from pre and post immunization. Healthy (male and females) controls were screened for the presence of mixed lymphocyte reaction blocking antibodies. The standard mixed lymphocyte reaction technique was used to evaluate the inhibitory effect of serum in the mixed lymphocyte reaction. Each serum was tested for cytotoxic antibodies. Immunoglobulin G and its isotypes were isolated according to the standard protocol. In the present study we have observed that there was significant inhibition of proliferation response when immunoglobulin G from different trimesters of pregnancy were added to one way mixed lymphocyte reaction or to phytohemagglutinin activated lymphocyte proliferation assay. Similar pattern was seen when immunoglobulin G isolated from adequately immunized women with recurrent spontaneous abortion was used. It was further confirmed that amongst all the isotypes of immunoglobulin G, only immunoglobulin G-3 was found to be positive for the inhibitory effect. Present study indicates that mixed lymphocyte reaction blocking antibodies are immunoglobulin G-3 in nature. It is developed during pregnancy and also after immunotherapy in women with recurrent spontaneous abortion who subsequently have the successful pregnancy.