Localization and synthesis of alphafoetoprotein in post-implantation mouse embryos.
Localization and synthesis of alphafoetoprotein in post-implantation mouse embryos.
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发表时间：
1978-02
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通讯作者：
By M. Dziadek;E. Adamson
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作者：
By M. Dziadek;E. Adamson
用制备的抗甲胎蛋白(AFP)抗血清，用免疫过氧化物酶和放射性标记后的免疫沉淀法研究了小鼠胚胎发育过程中AFP的定位和合成。甲胎蛋白在妊娠第7天的胚胎(第7天胚胎)中首次被检测到。在第7天和第8天的胚胎中，AFP仅局限于卵筒胚胎区周围的内脏(近端)内胚层细胞。内脏、胚胎外和壁层(远端)内胚层细胞不含甲胎蛋白。到妊娠第9天，AFP也出现在胚胎三腔周围的胚外胚层、中胚层和胚胎外胚层细胞中。这些组织在单独培养时不会合成甲胎蛋白，但当加入培养基时可以吸附甲胎蛋白。妊娠第12天，AFP合成仅限于内脏卵黄囊的内胚层。结论：AFP的合成能力仅限于植入后早期发育的内脏内胚层。甲胎蛋白在胚胎其他组织中的存在似乎是由于吸附所致。
The localization and synthesis of alphafoetoprotein (AFP) during mouse embryogenesis were studied by immunoperoxidase and by immunoprecipitation after radioactive labelling, using an antiserum prepared against AFP. AFP is first detectable in embryos on the 7th day of gestation (7th day embryos). In 7th and 8th day embryos AFP is confined to visceral (proximal) endoderm cells around the embryonic region of the egg cylinder. Visceral extra-embryonic and parietal (distal) endoderm cells do not contain AFP. By the 9th day of gestation AFP is also present in the extra-embryonic ectoderm, mesoderm and embryonic ectoderm cells around the three cavities of the embryo. These tissues do not synthesize AFP when cultured in isolation, but can adsorb AFP when it is added to the medium. On the 12th day of gestation AFP synthesis is confined to the endoderm layer of the visceral yolk sac. It is concluded that the ability to synthesize AFP is a property which is restricted to the visceral endoderm during early post-implantation development. The presence of AFP in other tissues of the embryo appears to be due to adsorption.