High-resolution analysis of gene rearrangements in lymphoid malignancies.
High-resolution analysis of gene rearrangements in lymphoid malignancies.
复制标题
淋巴恶性肿瘤基因重排的高分辨率分析。
DOI：
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复制
发表时间：
1999
期刊：
影响因子：
--
通讯作者：
H. Iland
中科院分区：
文献类型：
--
作者：
J. Ayling;H. Iland
As T-cell receptor and immunoglobulin gene rearrangements provide specific clonal markers for lymphoid cell proliferations, analysis of these genes is useful for distinguishing between reactive and malignant disease. We have developed an automated, high-resolution analysis of PCR fragments to identify clonally rearranged TCR-gamma (TCR gamma) genes and IgH genes. Consensus primers are used to detect the majority of possible rearrangements in multiplex PCR assays, and the PCR products are fluorescently labelled for visualisation with ABI Genescan software. Polyclonal populations of lymphoid cells are represented by a spectrum of fragments, whereas a monoclonal population of cells is represented by one or two discrete bands, indicating rearrangement of one or both alleles. For TCR-gamma PCR, the rearranged DNA fragment from a monoclonal population of T-cells diluted to 0.1% in DNA from a polyclonal population of cells is still readily distinguishable from the polyclonal background. Similarly, for IgH PCR, the gene rearrangement from a monoclonal population of B-cells is still distinguishable to 0.5% in a polyclonal background. As this technique allows semi-quantitative resolution of fragments one base different in size, it is ideal for detecting monoclonal and oligoclonal populations of B- and T-cells. The accurate size determination of PCR fragments also minimises the risk of false positives resulting from contamination, as individual monoclonal rearrangements are frequently patient-specific on the basis of size alone.
通过聚合酶链反应和变性梯度凝胶电泳 (PCR/DGGE) 检测早期蕈样肉芽肿/Sezary 综合征中克隆 T 细胞受体 γ 基因重排。
DOI：
10.1111/1523-1747.ep12389114
发表时间：
1994
期刊：
The Journal of investigative dermatology
影响因子：
--
作者：
Wood,GS;Tung,RM;Haeffner,AC;Crooks,CF;Liao,S;Orozco,R;Veelken,H;Kadin,ME;Koh,H;Heald,P
通讯作者：
Heald,P
人免疫球蛋白重链基因。
DOI：
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发表时间：
1989
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Capra,JD;Tucker,PW
通讯作者：
Tucker,PW