OsADK1, a novel kinase regulating arbuscular mycorrhizal symbiosis in rice
OsADK1, a novel kinase regulating arbuscular mycorrhizal symbiosis in rice
复制标题
OsADK1，一种调节水稻丛枝菌根共生的新型激酶
DOI：
10.1111/nph.17979
复制
发表时间：
2022-02-08
期刊：
影响因子：
9.4
通讯作者：
Wang, Bin
中科院分区：
文献类型：
--
作者：
Guo, Rui;Wu, Ya-Nan;Wang, Bin
丛枝菌根（AM）共生依赖于丛枝菌根的形成，在植物和AM真菌之间进行有效的养分交换。在这项研究中，我们在水稻中发现了一个新的名为OsADK1（丛枝发育激酶1）的激酶基因，该基因是丛枝发育所必需的。通过获得OsADK1pro::GUS转基因水稻植株，并通过CRISPR/Cas9技术产生Osadk1突变体，发现Osadk1在茎皮质细胞中被特异性诱导，Osadk1突变导致AM真菌Rhizophagus irregularis对水稻根系的定植率极低（约3%）。在突变根中，很少观察到的丛枝几乎全部在早期“树干形成”阶段停止，没有形成任何分支。增加AM真菌的接种量或与野生型护理植物共培养均未导致丛枝表型的恢复。对护理和未护理的Osadk1突变体的转录组测序显示，Osadk1突变可以极大地影响AM共生程序，包括许多关键转录因子，如RAM1和WRI5。因此，OsADK1代表了一种新的水稻激酶，它是丛枝分枝所必需的。它的鉴定为探索植物-真菌共生过程中决定丛枝发育的复杂信号转导途径打开了一扇新的窗口。
Arbuscular mycorrhizal (AM) symbiosis relies on the formation of arbuscules for efficient nutrient exchange between plants and AM fungi. In this study, we identified a novel kinase gene in rice named OsADK1 (Arbuscule Development Kinase 1) that is required for arbuscule development. By obtaining OsADK1pro::GUS transgenic rice plants and also generating Osadk1 mutants via CRISPR/Cas9 technique, OsADK1 was revealed to be specifically induced in the arbusculated cortical cells and mutations in OsADK1 resulted in an extremely low colonisation rate (c. 3%) of rice roots by AM fungus Rhizophagus irregularis. In the mutant roots, the very few observed arbuscules nearly all arrested at an early 'trunk-forming' phase without forming any branches. Increasing the inoculum strength of AM fungus or cocultivation with a wild-type nurse plant did not result in the rescue of the arbuscule phenotype. Transcriptome sequencing of both nursed and un-nursed Osadk1 mutants then revealed that the mutation of OsADK1 could greatly affect the AM symbiotic programme, including many key transcription factors such as RAM1 and WRI5. OsADK1 therefore represents a new rice kinase that is required for arbuscule branching. Its identification opens a new window to explore the elaborate signal transduction pathway that determines arbuscule development during plant-fungus symbiosis.