LIMITING DILUTION ANALYSIS OF THE B-CELL COMPARTMENT IN HUMAN-BONE MARROW
LIMITING DILUTION ANALYSIS OF THE B-CELL COMPARTMENT IN HUMAN-BONE MARROW
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DOI：
10.1002/eji.1830160206
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发表时间：
1986-02-01
影响因子：
5.4
通讯作者：
DOSCH, HM
中科院分区：
文献类型：
--
作者：
HIBI, T;DOSCH, HM
对成人肋骨标本的单个核细胞(MNC)体外产生免疫球蛋白(Ig)的能力进行了研究。用极限稀释法可以区分出3个B系细胞群体。第一种是在培养中产生免疫球蛋白，而不是任何故意的激活。这些细胞严格地不增殖，并维持5倍的超常分泌率。107-10次。107分子免疫球蛋白、免疫球蛋白A或免疫球蛋白M/细胞/小时，至少2周。它们以1倍的频率出现。10-4-10次。10-4(骨髓单个核细胞)或占骨髓B细胞的1-5%。在接触传染性EB病毒(EBV)后，这些细胞不会(A)增殖，(B)表达EB决定的核抗原(EBNA)，(C)提高其分泌速度或(D)长时间显示分泌活动。因此，这些细胞对EBV具有抗药性。如果这些细胞在体内以相似的速度产生抗体，那么它们的频率将表明它们可以提供高达每天合成免疫球蛋白和免疫球蛋白A的2/3和每天产生免疫球蛋白M的20-30%。第二批骨髓B细胞专门用于生产IgM。暴露于EV后，这些细胞增殖，表达EBNA，并开始分泌IgM。第三个群体占骨髓B细胞的90%。在我们手中，这些细胞在体外不能产生Ig。
Mononuclear cells (MNC) obtained from adult rib specimen were investigated for their capacity to produce immunoglobulin (Ig) in vitro. Using limiting dilution analysis 3 populations of B lineage cells could be distinguished. The first produces Ig in culture without any intentional activation. These cells are strictly nonproliferating and sustain extraordinary secretory rates of 5 .times. 107-10 .times. 107 molecules IgG, IgA or IgM/cell/h for at least 2 weeks. They occur at frequencies of 1 .times. 10-4-10 .times. 10-4 (marrow MNC) or represent 1-5% of marrow B cells. Following exposure to infectious Epstein-Bar virus (EBV), these cells do not (a) proliferate, (b) express EB-determined nuclear antigens (EBNA), (c) enhance their secretory rates or (d) show secretory activity for prolonged time. These cells are therefore EBV resistant. If these cells produced their antibody at similar rates in vivo, then their frequencies would suggest that they could provide up to 2/3 of daily synthetic rates for IgG and IgA and 20-30% of the daily IgM production. The second population of marrow B cells is exclusively committed to IgM production. Following exposure to EV these cells proliferate, express EBNA and begin to secrete IgM. The third population represents 90% of marrow B cells. In our hands, these cells are unable to produce Ig in vitro.