Multiplexed Luminescence Oxygen Channeling Immunoassay Based on Dual-Functional Barcodes with a Host-Guest Structure: A Facile and Robust Suspension Array Platform
Multiplexed Luminescence Oxygen Channeling Immunoassay Based on Dual-Functional Barcodes with a Host-Guest Structure: A Facile and Robust Suspension Array Platform
复制标题
基于具有主客体结构的双功能条形码的多重发光氧通道免疫分析：一个简便而稳健的悬浮阵列平台
DOI：
10.1002/smll.201907521
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发表时间：
2020-04-01
期刊：
影响因子：
13.3
通讯作者：
Gu, Hongchen
中科院分区：
文献类型：
--
作者：
Guo, Qingsheng;Wang, Yao;Gu, Hongchen
开发一个功能强大的免疫分析平台，具有简单和高复用的能力，是有希望的疾病诊断。为了满足这一迫切需求，首次报道了通过悬浮阵列技术实现LOCI的多重发光氧通道免疫分析（multi-LOCI）平台。设计了一种独特的双功能条形码作为平台的微载体，该条形码由量子点宿主珠（QDH）和LOCI受体珠（AB s）组成，具有主客体结构，其中QDH提供高编码能力，AB s促进LOCI功能。分析物将QDH@ AB和LOCI供体珠（DB）桥接成紧密接近，形成QDH@ ABs-DB“主体-客体-卫星”超结构，其产生来自QDH的条形码信号和由AB和DB之间的单线态氧通道诱导的LOCI信号。通过基于成像的解码，不同的条形码被自动区分并与LOCI信号共定位。重要的是，该分析实现了在一个反应中同时检测多种分析物，只需遵循“混合和测量”方案，而无需繁琐的洗涤步骤。此外，多LOCI平台被验证用于真实的样品测量。该平台具有鲁棒性、简单性和高度多路复用的优点，具有发展即时诊断的巨大潜力。
The development of a powerful immunoassay platform with capacities of both simplicity and high multiplexing is promising for disease diagnosis. To meet this urgent need, for the first time, a multiplexed luminescent oxygen channeling immunoassay (multi-LOCI) platform by implementation of LOCI with suspension array technology is reported. As the microcarrier of the platform, a unique dual-functional barcode with a host-guest structure composed of a quantum dot host bead (QDH) and LOCI acceptor beads (ABs) is designed, in which QDH provides function of high coding capacity while ABs facilitate the LOCI function. The analytes bridge QDH@ABs and LOCI donor beads (DBs) into a close proximity, forming a QDH@ABs-DBs "host-guest-satellite" superstructure that generates both barcode signal from QDH and LOCI signal induced by singlet oxygen channeling between ABs and DBs. Through imaging-based decoding, different barcodes are automatically distinguished and colocalized with LOCI signals. Importantly, the assay achieves simultaneous detection of multiple analytes within one reaction, simply by following a "mix-and-measure" protocol without the need for tedious washing steps. Furthermore, the multi-LOCI platform is validated for real sample measurements. With the advantages of robustness, simplicity, and high multiplexing, the platform holds great potential for the development of point-of-care diagnostics.