Auxin-dependent control of a plasmodesmal regulator creates a negative feedback loop modulating lateral root emergence
Auxin-dependent control of a plasmodesmal regulator creates a negative feedback loop modulating lateral root emergence
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DOI：
10.1038/s41467-019-14226-7
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发表时间：
2020-01-17
影响因子：
16.6
通讯作者：
Lee, Jung-Youn
中科院分区：
文献类型：
--
作者：
Sager, Ross;Wang, Xu;Lee, Jung-Youn
侧根起源于主根深处的原始细胞，必须通过几个覆盖层才能出现。侧根的出现需要新原基（LRP）的生长与上覆根细胞的及时分离相一致，这是一个由激素生长素协调的发育程序。在这里，我们报告说，在拟南芥根，生长素控制的时空表达的胞间连丝调节PDLP 5的细胞覆盖LRP，创造一个负反馈回路。PDLP5的功能是通过胞间连丝限制信号在细胞间的移动，它是由生长素在细胞中以渐进的方式覆盖LRP诱导的。PDLP 5定位于这些细胞中的胞间连丝，并对器官出现以及整个根分支产生负面影响。我们提出了一个模型，将时空表达的PDLP5在LRP覆盖的细胞到已知的生长素调节的LRP覆盖的细胞分离途径，并推测PDLP5可能发挥作用，负调控侧根出现的过程。生长素促进侧根从根脉管系统中的周鞘细胞中出现。在这里，作者表明，在侧根出现过程中诱导的胞间连丝调节因子PDLP 5限制了生长素信号传导到原基上的细胞的空间范围。
Lateral roots originate from initial cells deep within the main root and must emerge through several overlying layers. Lateral root emergence requires the outgrowth of the new primordium (LRP) to coincide with the timely separation of overlying root cells, a developmental program coordinated by the hormone auxin. Here, we report that in Arabidopsis thaliana roots, auxin controls the spatiotemporal expression of the plasmodesmal regulator PDLP5 in cells overlying LRP, creating a negative feedback loop. PDLP5, which functions to restrict the cell-to-cell movement of signals via plasmodesmata, is induced by auxin in cells overlying LRP in a progressive manner. PDLP5 localizes to plasmodesmata in these cells and negatively impacts organ emergence as well as overall root branching. We present a model, incorporating the spatiotemporal expression of PDLP5 in LRP-overlying cells into known auxin-regulated LRP-overlying cell separation pathways, and speculate how PDLP5 may function to negatively regulate the lateral root emergence process. Auxin promotes lateral root emergence from pericycle cells in the root vasculature. Here the authors show that induction of the plasmodesmal regulator PDLP5 during lateral root emergence restricts the spatial scope of auxin signaling to the cells overlying the primordia.