Cloning and characterization of an anaerobically induced cDNA encoding glucose-6-phosphate isomerase from maize.
Cloning and characterization of an anaerobically induced cDNA encoding glucose-6-phosphate isomerase from maize.
复制标题
编码玉米葡萄糖-6-磷酸异构酶的厌氧诱导 cDNA 的克隆和表征。
DOI：
10.1104/pp.108.3.1295
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发表时间：
1995
期刊：
影响因子：
7.4
通讯作者：
Sachs,MM
中科院分区：
文献类型：
--
作者：
Lal,SK;Sachs,MM
玉米（Zea mays L.）的厌氧胁迫导致基因表达的剧烈改变，并导致少量被称为厌氧蛋白的多肽的选择性合成（Sachs et al., 1980）。其他被研究的植物在受到厌氧环境时也表现出类似的反应（Russell et al., 1990）。迄今为止，大多数已确定的厌氧蛋白被发现是参与糖酵解途径或葡萄糖- p代谢的酶。然而，最近Peschke和Sachs（1994）报道了两种厌氧诱导的转录本，它们不编码参与葡萄糖代谢的酶，显然在玉米对洪水胁迫的反应中起着其他作用。厌氧蛋白的表达被认为在转录和翻译两个层面受到调控（Sachs et al., 1980; Hake et al., 1985）。Gpi （ec 5.3）1.9)是一种必需的糖酵解酶，它催化Glc-6-P和Fru-6-P的可逆异构化，这一反应也立即发生在植物中Suc生物合成之前。这种酶也被称为磷酸己糖异构酶和磷酸葡萄糖异构酶。GPI的两种同工酶形式，一种在细胞质中，另一种在质体中，已经在其他植物中报道过，也可能存在于玉米中（Weeden and Gottlieb, 1982; Kelley and Freeling, 1984）。一般来说，被检测的植物含有一个GPI-C基因，但克氏菌除外，它有两个拷贝，这是由于该物种内部的基因复制造成的（Weeden和Gottlieb， 1979）。据报道，玉米中有三种形式的转基因（Salamini等人，1972年）。由phil基因编码的gpi - 1在两种细胞中均有表达
Anaerobic stress in maize (Zea mays L.) leads to a drastic alteration in gene expression and results in the selective synthesis of a small number of polypeptides named anaerobic proteins (Sachs et al., 1980). Other plants studied showed similar responses when subjected to an anaerobic environment (Russell et al., 1990). To date, the majority of the anaerobic proteins that have been identified were found to be enzymes involved in the glycolytic pathway or Glc-P metabolism. Recently, however, Peschke and Sachs (1994) reported two anaerobically induced transcripts that do not encode enzymes involved in Glc metabolism and apparently serve other roles in the response of maize to flooding stress. The expression of the anaerobic proteins has been proposed to be regulated at the levels of both transcription and translation (Sachs et al., 1980; Hake et al., 1985).GPI (EC 5.3. 1.9) is an essential glycolytic enzyme that catalyzes the reversible isomerization of Glc-6-P and Fru-6-P, a reaction that also immediately precedes Suc biosynthesis in plants. This enzyme has also been called phosphohexose isomerase and phophoglucose isomerase. Two isozyme forms of GPI, one localized in the cytosol and the other in the plastid, have been reported in other plants and may also exist in maize (Weeden and Gottlieb, 1982; Kelley and Freeling, 1984). In general, plants examined contain a single gene for GPI-C, with the exception of Clarkia, which has two copies, resulting from gene duplication within that species (Weeden and Gottlieb, 1979). Three forms of GM have been reported in maize (Salamini et al., 1972). GPI-I encoded by the phil gene is expressed in both the devel-